Cloned (Comment) | Organism |
---|---|
recombinant expression of 17beta-HSD14 wild-type variants S205 and T205 in Escherichia coli strain BL21 pLysS | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
the purified enzyme variants T205 and S205 in apoform, as holoenzyme, or in complex with estrone or inhibitor [6-(3,4-dihydroxyphenyl)pyridin-2-yl]-(4-fluoro-3-hydroxyphenyl)methanone, ternary complex enzyme-cofactor-inhibitor, X-ray diffraction structure determination and analysis at 1.52-2.02 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S205T | natural genetic polymorphism | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
[6-(3,4-dihydroxyphenyl)pyridin-2-yl]-(4-fluoro-3-hydroxyphenyl)methanone | a potent nonsteroidal inhibitor, binding structure, overview | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
0.00062 | - |
17beta-estradiol | 17beta-HSD14 variant S205, pH and temperature not specified in the publication | Homo sapiens | |
0.00079 | - |
17beta-estradiol | 17beta-HSD14 variant T205, pH and temperature not specified in the publication | Homo sapiens | |
0.0066 | - |
5-androstene-3beta,17beta-diol | 17beta-HSD14 variant S205, pH and temperature not specified in the publication | Homo sapiens | |
0.0078 | - |
5-androstene-3beta,17beta-diol | 17beta-HSD14 variant T205, pH and temperature not specified in the publication | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-estradiol + NAD+ | Homo sapiens | - |
estrone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9BPX1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adrenal gland | - |
Homo sapiens | - |
brain | - |
Homo sapiens | - |
breast cancer cell | - |
Homo sapiens | - |
esophagus | - |
Homo sapiens | - |
eye | - |
Homo sapiens | - |
heart | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
placenta | - |
Homo sapiens | - |
rectum | - |
Homo sapiens | - |
salivary gland | - |
Homo sapiens | - |
skeletal muscle | - |
Homo sapiens | - |
testis | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17beta-estradiol + NAD+ | - |
Homo sapiens | estrone + NADH + H+ | - |
r | |
5-androstene-3beta,17beta-diol + NAD+ | - |
Homo sapiens | ? + NADH + H+ | - |
? | |
testosterone + NAD+ | - |
Homo sapiens | androst-4-ene-3,17-dione + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
17beta-HSD14 | - |
Homo sapiens |
17beta-hydroxysteroid dehydrogenase type 14 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00028 | - |
5-androstene-3beta,17beta-diol | 17beta-HSD14 variant S205, pH and temperature not specified in the publication | Homo sapiens | |
0.00032 | - |
5-androstene-3beta,17beta-diol | 17beta-HSD14 variant T205, pH and temperature not specified in the publication | Homo sapiens | |
0.0004 | - |
17beta-estradiol | 17beta-HSD14 variant S205, pH and temperature not specified in the publication | Homo sapiens | |
0.00055 | - |
17beta-estradiol | 17beta-HSD14 variant T205, pH and temperature not specified in the publication | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Homo sapiens | |
NADH | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000007 | - |
[6-(3,4-dihydroxyphenyl)pyridin-2-yl]-(4-fluoro-3-hydroxyphenyl)methanone | pH and temperature not specified in the publication | Homo sapiens |