Application | Comment | Organism |
---|---|---|
analysis | dipstick assay for the detection of gamma-hydroxybutyrate in alcoholic beverages | Cupriavidus necator |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli pLysS as stable fusion protein | Cupriavidus necator |
General Stability | Organism |
---|---|
addition of ammonium sulfate, glycerol, 2-mercaptoethanol, dithiothreitol, glutathione, or EDTA reduces activity during storage | Cupriavidus necator |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.19 | - |
gamma-hydroxybutyrate | pH 8.5, 23°C | Cupriavidus necator | |
14 | - |
ethanol | pH 8.5, 23°C | Cupriavidus necator |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40500 | - |
calculated from the predicted amino acid sequence of the native enzyme | Cupriavidus necator |
66000 | - |
fusion protein, SDS-PAGE, calculated mass | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybutanoate + NADP+ | Cupriavidus necator | - |
succinate semialdehyde + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxybutanoate + NADP+ | - |
Cupriavidus necator | succinate semialdehyde + NADPH + H+ | - |
? | |
ethanol + NADP+ | - |
Cupriavidus necator | ethanal + NADPH + H+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
-80 | - |
stable for indefinite timespan | Cupriavidus necator |
4 | - |
stable for at least 21 days, 60% of activity after 3.5 months | Cupriavidus necator |