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Literature summary for 1.1.1.6 extracted from

  • Musille, P.; Ortlund, E.
    Structure of glycerol dehydrogenase from Serratia (2014), Acta Crystallogr. Sect. F, 70, 166-172.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.6

Cloned(Commentary)

Cloned (Comment) Organism
cloning from contaminating bacteria, DNA and amino acid sequence determmination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli train BL21(DE3)pLysS Serratia sp.

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion, mixing of 0.001 ml 6 mg/ml protein in 150 mM ammonium acetate, 50 mM sodium chloride, 20 mM Tris, pH 7.4, 5% glycerol with 0.001 ml reservoir solution containing 5-10% PEG 3350, 0.2 M calcium acetate, and 4% glycerol, X-ray diffraction structure determination and analysis at 1.90 A resolution, molecular replacement using PDB entry 1jpu as a search model Serratia sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ active-site zinc responsible for coordinating glycerol in the active site of the enzyme. The active site is highly conserved and contains a zinc ion coordinated by two histidines and an aspartate Serratia sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
-
 Serratia sp.
160000
-
 recombinant His6-tagged enzyme, ge filtration Serratia sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycerol + NAD+ Serratia sp.
-
 glycerone + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Serratia sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged enzyme from Escherichia coli train BL21(DE3)pLysS by nicke affinity chromatography and gel filtration Serratia sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycerol + NAD+
-
 Serratia sp. glycerone + NADH + H+
-
 r

Subunits

Subunits Comment Organism
More protein structure-function relationships Serratia sp.
tetramer 4 * 37000, recombinant His6-tagged enzyme, SDS-PAGE Serratia sp.

Synonyms

Synonyms Comment Organism
GDH
-
 Serratia sp.

Cofactor

Cofactor Comment Organism Structure
NAD+ enzyme binding structure, overview Serratia sp.
NADH
-
 Serratia sp.

General Information

General Information Comment Organism
additional information protein structure-function relationships Serratia sp.
physiological function glycerol dehydrogenase is the enzyme responsible for the oxidation of glycerol to dihydroxyacetone. This permits its entrance into the glycolytic pathway Serratia sp.