Activating Compound | Comment | Organism | Structure |
---|---|---|---|
NH4+ | - |
Cellulomonas sp. |
Crystallization (Comment) | Organism |
---|---|
- |
Cellulomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dihydroxyacetone | noncompatible inhibitor, at saturated level of glycerol | Cellulomonas sp. | |
Hydroxyacetone | - |
Cellulomonas sp. | |
NADH | - |
Cellulomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.385 | - |
dihydroxyacetone | - |
Cellulomonas sp. | |
10.9 | - |
glycerol | - |
Cellulomonas sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | soluble, isolation from crude cell extract | Cellulomonas sp. | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | oxidation of glycerol os greatly increased | Cellulomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
336000 | 390000 | gel filtration | Cellulomonas sp. |
336000 | 390000 | sedimentation equilibrium method | Cellulomonas sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Cellulomonas sp. | - |
dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | Cellulomonas sp. NT3060 | - |
dihydroxyacetone + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cellulomonas sp. | - |
- |
- |
Cellulomonas sp. NT3060 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Cellulomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
128 | - |
- |
Cellulomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-propanediol + NAD+ | - |
Cellulomonas sp. | hydroxyacetone + NADH | - |
r | |
1,2-propanediol + NAD+ | - |
Cellulomonas sp. NT3060 | hydroxyacetone + NADH | - |
r | |
2,3-butanediol + NAD+ | - |
Cellulomonas sp. | 3-hydroxybutane-2-one + NADH | - |
r | |
2,3-butanediol + NAD+ | - |
Cellulomonas sp. NT3060 | 3-hydroxybutane-2-one + NADH | - |
r | |
glycerol + NAD+ | - |
Cellulomonas sp. | dihydroxyacetone + NADH + H+ | - |
? | |
glycerol + NAD+ | - |
Cellulomonas sp. NT3060 | dihydroxyacetone + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | 8 * 42000-43000, SDS-PAGE | Cellulomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
oxidation of glycerol | Cellulomonas sp. |
50 | - |
oxidation of butanediol | Cellulomonas sp. |
65 | - |
oxidation of propanediol | Cellulomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
reduction of hydroxyacetone | Cellulomonas sp. |
6 | - |
reduction of dihydroxyacetone or 3-hydroxybutane-2-one | Cellulomonas sp. |
8 | 8.5 | oxidation of 1,2-propanediol | Cellulomonas sp. |
9 | - |
oxidation of glycerol or butanediol | Cellulomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cellulomonas sp. | |
NADH | - |
Cellulomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
11.1 | - |
dihydroxyacetone | - |
Cellulomonas sp. | |
11.6 | - |
Hydroxyacetone | - |
Cellulomonas sp. |