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Literature summary for 1.1.1.6 extracted from
- Purification and properties of glycerol dehydrogenase from Candida valida (1984), J. Gen. Microbiol., 130, 3225-3233.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| citrate | the enzyme is more active in presence of multivalent anions, citrate, phosphate or sulfate, in comparison to monovalent anions, e.g. acetate or chloride | Pichia membranifaciens |  |
| phosphate | the enzyme is more active in presence of multivalent anions, citrate, phosphate or sulfate, in comparison to monovalent anions, e.g. acetate or chloride | Pichia membranifaciens | |
| SO42- | the enzyme is more active in presence of multivalent anions, citrate, phosphate or sulfate, in comparison to monovalent anions, e.g. acetate or chloride | Pichia membranifaciens | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-amino-2-(hydroxymethyl)-1,3-propanediol | strong inhibition, IC50 2 mM | Pichia membranifaciens | |
| 2-mercaptoethanol | strong inhibition, IC50 0.3 mM | Pichia membranifaciens | |
| dihydroxyacetone phosphate | - |
Pichia membranifaciens | |
| dithiothreitol | slight inhibition | Pichia membranifaciens | |
| ethylene glycol | strong inhibition, IC50 4 mM | Pichia membranifaciens | |
| glycerol 3-phosphate | - |
Pichia membranifaciens | |
| L-cysteine | slight inhibition | Pichia membranifaciens | |
| N-ethylmaleimide | rapid inactivation, IC50: 0.2 mM | Pichia membranifaciens | |
| NAD+ | - |
Pichia membranifaciens | |
| NADH | product inhibition , competitive with NAD+ | Pichia membranifaciens | |
| NADP+ | - |
Pichia membranifaciens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.048 | - |
NADH | 25°C | Pichia membranifaciens | |
| 0.14 | - |
NAD+ | 25°C | Pichia membranifaciens | |
| 0.77 | - |
dihydroxyacetone | 25°C | Pichia membranifaciens | |
| 58 | - |
glycerol | 25°C | Pichia membranifaciens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | soluble, isolation from crude cell extract | Pichia membranifaciens | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Pichia membranifaciens |
| 76000 | 80000 | gel filtration, PAGE under nondenaturing conditions | Pichia membranifaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol + NAD+ | Pichia membranifaciens | - |
dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | Pichia membranifaciens H 112 | - |
dihydroxyacetone + NADH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pichia membranifaciens | - |
- |
- |
| Pichia membranifaciens H 112 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pichia membranifaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 448 | - |
- |
Pichia membranifaciens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -15°C, with 5% culture filtrate or 5% boiled crude cell extract, 10% Ficoll, pH 7.5, stable for 1 month | Pichia membranifaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,2-propanediol + NAD+ | - |
Pichia membranifaciens | hydroxyacetone + NADH | - |
? | |
| 1,2-propanediol + NAD+ | - |
Pichia membranifaciens H 112 | hydroxyacetone + NADH | - |
? | |
| DL-glyceraldehyde + NAD+ | - |
Pichia membranifaciens | 3-hydroxypyruvaldehyde + NADH | - |
? | |
| DL-glyceraldehyde + NAD+ | - |
Pichia membranifaciens H 112 | 3-hydroxypyruvaldehyde + NADH | - |
? | |
| glycerol + NAD+ | - |
Pichia membranifaciens | dihydroxyacetone + NADH + H+ | - |
? | |
| glycerol + NAD+ | - |
Pichia membranifaciens H 112 | dihydroxyacetone + NADH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 38000, SDS-PAGE | Pichia membranifaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 36 | - |
- |
Pichia membranifaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8.5 | reduction of dihydroxyacetone, phosphate buffer | Pichia membranifaciens |
| 8 | - |
oxidation of glycerol, tetraethyl ammonium chloride/tetraethylammonium hydroxide buffer | Pichia membranifaciens |
| 9.5 | 10.5 | oxidation of glycerol, diphosphate buffer | Pichia membranifaciens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
most stable | Pichia membranifaciens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Pichia membranifaciens | |
| NADH | - |
Pichia membranifaciens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.025 | - |
NADH | 25°C | Pichia membranifaciens | |
| 1.09 | - |
NAD+ | 25°C | Pichia membranifaciens | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
rapid inactivation, IC50: 0.2 mM | Pichia membranifaciens | N-ethylmaleimide | |
| 0.3 | - |
strong inhibition, IC50 0.3 mM | Pichia membranifaciens | 2-mercaptoethanol | |
| 2 | - |
strong inhibition, IC50 2 mM | Pichia membranifaciens | 2-amino-2-(hydroxymethyl)-1,3-propanediol | |
| 4 | - |
strong inhibition, IC50 4 mM | Pichia membranifaciens | ethylene glycol | |
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