Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) | Bacillus cereus |
MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha | Bacillus cereus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ZnCl2 | 0.2 mM, 60% inhibition | Bacillus cereus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetic analysis, overview | Bacillus cereus | |
0.25 | - |
NADP+ | pH 8.5, 37°C | Bacillus cereus | |
2.4 | - |
NAD+ | pH 8.5, 37°C | Bacillus cereus | |
16.8 | - |
3-hydroxypropanoate | pH 8.5, 37°C | Bacillus cereus | |
16.8 | - |
3-hydroxypropionate | pH 8.5, 37°C, KM-value for 3-hydroxyisobutyrate is approximately 20fold lower | Bacillus cereus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no metal ion requirement | Bacillus cereus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Bacillus cereus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxypropanoate + NAD+ | Bacillus cereus | - |
3-oxopropanoate + NADH + H+ | - |
r | |
additional information | Bacillus cereus | MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus cereus | - |
- |
- |
Bacillus cereus | - |
MmsB gene | - |
Bacillus cereus ATCC 14579 | - |
- |
- |
Bacillus cereus ATCC 14579 | - |
MmsB gene | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography | Bacillus cereus |
native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography | Bacillus cereus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.77 | - |
pH 8.5, 37°C | Bacillus cereus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxyisobutyrate + NAD+ | - |
Bacillus cereus | methylmalonate semialdehyde + NADH + H+ | - |
r | |
3-hydroxypropanoate + NAD+ | - |
Bacillus cereus | 3-oxopropanoate + NADH + H+ | - |
r | |
3-hydroxypropanoate + NAD+ | - |
Bacillus cereus | malonate semialdehyde + NADH + H+ | - |
r | |
3-hydroxypropanoate + NADP+ | - |
Bacillus cereus | malonate semialdehyde + NADPH + H+ | - |
r | |
additional information | MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity | Bacillus cereus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Bacillus cereus |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxyisobutyrate dehydrogenase | cf. EC1.1.1.31, wide substrate specificity (hydroxyacid derivatives) | Bacillus cereus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Bacillus cereus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 45 | 90% of maximal activity within this range | Bacillus cereus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
30 min, purified enzyme, completely stable | Bacillus cereus |
45 | - |
stable at 45°C for 30 min, about 40% activity after 3 min at 55°C | Bacillus cereus |
55 | - |
3 min, purified enzyme, significant denaturation and inactivation | Bacillus cereus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
3-hydroxypropionate | pH 8.5, 37°C | Bacillus cereus | |
0.21 | - |
3-hydroxypropanoate | pH 8.5, 37°C | Bacillus cereus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | 9 | - |
Bacillus cereus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | - |
Bacillus cereus |
7 | 10 | activity range | Bacillus cereus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus cereus | |
NADH | - |
Bacillus cereus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0125 | - |
3-hydroxypropionate | pH 8.5, 37°C | Bacillus cereus | |
0.0125 | - |
3-hydroxypropanoate | pH 8.5, 37°C | Bacillus cereus |