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Literature summary for 1.1.1.57 extracted from
- Uronic acid metabolism in bacteria. II Purification and properties of D-altronic acid and D-mannonic acid dehydrogenase in Escherichia coli (1960), J. Biol. Chem., 235, 1566-1570.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Escherichia coli |  |
| EDTA | - |
Escherichia coli | |
General Stability
| General Stability | Organism |
|---|---|
| rapid loss of activity upon dialysis and mild heating | Escherichia coli |
| stabilized by 1 mM cysteine at pH 7 | Escherichia coli |
| unstable on freezing and thawing | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| p-chloromercuribenzoate | complete inhibition at 0.03 mM | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1 | - |
D-fructuronate | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-mannonate + NAD+ | Escherichia coli | - |
D-fructuronate + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 26 | - |
crude extract | Escherichia coli |
| 770 | - |
purified enzyme | Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 3°C, pH 7, 1 mM cysteine, stable for 1-2 weeks | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-altronate + NAD+ | approximately equal activity towards both fructuronate and tagaturonate in the forward reaction, and towards D-mannonate and D-altronate in the reverse reaction | Escherichia coli | D-tagaturonate + NADH | - |
r | |
| D-mannonate + NAD+ | - |
Escherichia coli | D-fructuronate + NADH | - |
r | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Escherichia coli |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
unstable at lower pH | Escherichia coli |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Escherichia coli | |
| NADH | - |
Escherichia coli | |
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