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Literature summary for 1.1.1.56 extracted from

  • Hassanin, H.A.; Wang, X.; Mu, W.; Zhang, T.; Jiang, B.
    Cloning and characterization of a new ribitol dehydrogenase from Providencia alcalifaciens RIMD 1656011 (2016), J. Sci. Food Agric., 96, 2917-2924.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Providencia alcalifaciens

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ about 20% residual activity at 1 mM Providencia alcalifaciens
Zn2+ about 43% residual activity at 1 mM Providencia alcalifaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.042
-
NAD+ at pH 10.0 and 35°C Providencia alcalifaciens
13.9
-
ribitol at pH 10.0 and 35°C Providencia alcalifaciens

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ about 126.8% activity at 1 mM Providencia alcalifaciens
Ca2+ about 113.5% activity at 1 mM Providencia alcalifaciens
Co2+ about 111% activity at 1 mM Providencia alcalifaciens
Mg2+ about 114.8% activity at 1 mM Providencia alcalifaciens
additional information not influenced by Mn2+ Providencia alcalifaciens
Ni2+ about 108% activity at 1 mM Providencia alcalifaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
4 * 25000, SDS-PAGE Providencia alcalifaciens
26650
-
-
Providencia alcalifaciens
104000
-
gel filtration Providencia alcalifaciens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribitol + NAD+ Providencia alcalifaciens high activity D-ribulose + NADH + H+
-
?
ribitol + NAD+ Providencia alcalifaciens RIMD 1656011 high activity D-ribulose + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Providencia alcalifaciens
-
-
-
Providencia alcalifaciens RIMD 1656011
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel-charged Sepharose resin column chromatography Providencia alcalifaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-psicose + NADH + H+
-
Providencia alcalifaciens allitol + NAD+
-
?
D-psicose + NADH + H+
-
Providencia alcalifaciens RIMD 1656011 allitol + NAD+
-
?
additional information the enzyme shows 8.6% activity with allitol, low activity with xylitol (1.8%) and no activity with D-sorbitol, L-arabitol, galactitol, D-mannitol, inositol and glycerol Providencia alcalifaciens ?
-
?
additional information the enzyme shows 8.6% activity with allitol, low activity with xylitol (1.8%) and no activity with D-sorbitol, L-arabitol, galactitol, D-mannitol, inositol and glycerol Providencia alcalifaciens RIMD 1656011 ?
-
?
ribitol + NAD+ high activity Providencia alcalifaciens D-ribulose + NADH + H+
-
?
ribitol + NAD+ high activity Providencia alcalifaciens RIMD 1656011 D-ribulose + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 25000, SDS-PAGE Providencia alcalifaciens
homotetramer 4 * 25928, calculated from amino acid sequence Providencia alcalifaciens
homotetramer 4 * 26650, liquid chromatography/mass spectrometry Providencia alcalifaciens

Synonyms

Synonyms Comment Organism
RDH
-
Providencia alcalifaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Providencia alcalifaciens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 45 about 90% activity at 25°C, 100% activity at 35°C, about 80% activity at 40°C, about 55% activity at 45°C Providencia alcalifaciens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25 50 the enzyme retains 72, 72, 48 and 0% of its initial activity after 4 h at 25, 30, 40 and 50°C, respectively Providencia alcalifaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10
-
ribitol at pH 10.0 and 35°C Providencia alcalifaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
-
Providencia alcalifaciens

pH Range

pH Minimum pH Maximum Comment Organism
9 11 the enzyme shows about 25% activity at pH 9.0, while at pH 10.5 and 11.0 the activity is 85.6 and 68.11% respectively of that at pH 10.0 Providencia alcalifaciens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Providencia alcalifaciens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.71
-
ribitol at pH 10.0 and 35°C Providencia alcalifaciens