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Literature summary for 1.1.1.56 extracted from

  • Singh, R.; Singh, R.; Kim, I.W.; Sigdel, S.; Kalia, V.C.; Kang, Y.C.; Lee, J.K.
    An efficient ribitol-specific dehydrogenase from Enterobacter aerogenes (2015), Enzyme Microb. Technol., 72, 56-64.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells Klebsiella aerogenes

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 50.5% residual activity at 5 mM Klebsiella aerogenes
EDTA the enzyme activity is mostly lost (about 97%) after EDTA treatment Klebsiella aerogenes
Fe3+ 27.2% residual activity at 5 mM Klebsiella aerogenes
Hg2+ complete inhibition at 1 mM Klebsiella aerogenes
K+ 73.6% residual activity at 5 mM Klebsiella aerogenes
Zn2+ 61.9% residual activity at 5 mM Klebsiella aerogenes

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
NAD+ at pH 11.0 and 25°C Klebsiella aerogenes
10.3
-
ribitol at pH 11.0 and 25°C Klebsiella aerogenes

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 209% activity at 5 mM Klebsiella aerogenes
Ca2+ 245% activity at 5 mM Klebsiella aerogenes
Mg2+ 162% activity at 5 mM Klebsiella aerogenes
additional information not affected by Mn2+ and Co2+ Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25800
-
-
Klebsiella aerogenes
110000
-
gel filtration Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ribitol + NAD+ Klebsiella aerogenes
-
D-ribulose + NADH + H+
-
?
ribitol + NAD+ Klebsiella aerogenes KCTC 2190
-
D-ribulose + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes A0A0H3FYM1
-
-
Klebsiella aerogenes KCTC 2190 A0A0H3FYM1
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with NADP+ Klebsiella aerogenes ?
-
?
additional information no activity with NADP+ Klebsiella aerogenes KCTC 2190 ?
-
?
ribitol + NAD+
-
Klebsiella aerogenes D-ribulose + NADH + H+
-
?
ribitol + NAD+
-
Klebsiella aerogenes KCTC 2190 D-ribulose + NADH + H+
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 25800, calculated from amino acid sequence Klebsiella aerogenes

Synonyms

Synonyms Comment Organism
NAD+-dependent ribitol dehydrogenase
-
Klebsiella aerogenes
RDH
-
Klebsiella aerogenes
ribitol-specific dehydrogenase
-
Klebsiella aerogenes
ribitol: NAD+ 2-oxidoreductase
-
Klebsiella aerogenes

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Klebsiella aerogenes

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 60 the enzyme retains more than 90% of the maximum activity at 50°C, with more than 85% activity at 55°C, and more than 80% at 60°C Klebsiella aerogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40 60 the enzyme shows half-lives of 25.7, 15.3, 7.5, 3.1, and 0.45 h at 40°C, 45°C, 50°C, 55°C, and 60°C, respectively Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
Klebsiella aerogenes

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Klebsiella aerogenes

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
30.9
-
ribitol at pH 11.0 and 25°C Klebsiella aerogenes