Protein Variants | Comment | Organism |
---|---|---|
G196P | mutant D, increased activity towards xylitol | Klebsiella aerogenes |
General Stability | Organism |
---|---|
half life in crude extract at 55°C mutant B: 6 min | Klebsiella aerogenes |
half life in crude extract at 55°C mutant D: 30 min | Klebsiella aerogenes |
half life in crude extract at 55°C wild type: 20 min | Klebsiella aerogenes |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ribitol dehydrogenase A and B | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribitol + NAD+ | - |
Klebsiella aerogenes | D-ribulose + NADH | - |
? | |
xylitol + NAD+ | mutants have increased xylitol activity | Klebsiella aerogenes | D-xylulose + NADH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ribitol dehydrogenase A | wild type | Klebsiella aerogenes |
ribitol dehydrogenase B | mutant enzyme with different properties | Klebsiella aerogenes |
ribitol dehydrogenase D | mutant enzyme with different properties | Klebsiella aerogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life of mutant B is 6 min, half-life of mutant D is 30 min, half-life of wild-type enzyme is 20 min | Klebsiella aerogenes |