Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)pLysS cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
in complex with NADP+ and progesterone, sitting drop vapor diffusion method, using 100 mM sodiumcacodylate buffer (pH 6.0), 200 mM ammonium sulfate, 24-26% (w/v) polyethylene glycol 3350 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
V54L | the mutation restricts the steroid alternative binding and enhances the 20alpha-hydroxysteroid dehydrogenase activity of the enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00084 | - |
progesterone | wild type enzyme, at pH 7.4 and 37°C | Homo sapiens | |
0.00213 | - |
progesterone | mutant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
0.00218 | - |
5alpha-dihydrotestosterone | wild type enzyme, at pH 7.4 and 37°C | Homo sapiens | |
0.07103 | - |
5alpha-dihydrotestosterone | mutant enzyme, at pH 7.4 and 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione agarose column chromatography and Q-Sepharose column chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5alpha-dihydrotestosterone + NADP+ | - |
Homo sapiens | 5alpha-androstane-3,17-dione + NADPH + H+ | - |
? | |
progesterone + NADP+ | - |
Homo sapiens | 20beta-hydroxy-pregn-4-ene-3-one + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-alpha hydroxysteroid dehydrogenase type 3 | - |
Homo sapiens |
3alpha-HSD3 | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
progesterone | wild type enzyme, at pH 7.4 and 37°C | Homo sapiens | |
0.039 | - |
progesterone | mutant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
0.061 | - |
5alpha-dihydrotestosterone | wild type enzyme, at pH 7.4 and 37°C | Homo sapiens | |
0.115 | - |
5alpha-dihydrotestosterone | mutant enzyme, at pH 7.4 and 37°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
100 | - |
5alpha-dihydrotestosterone | mutant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
250 | - |
progesterone | wild type enzyme, at pH 7.4 and 37°C | Homo sapiens | |
1100 | - |
progesterone | mutant enzyme, at pH 7.4 and 37°C | Homo sapiens | |
1680 | - |
5alpha-dihydrotestosterone | wild type enzyme, at pH 7.4 and 37°C | Homo sapiens |