Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.53 extracted from

  • Guan, G.; Todo, T.; Tanaka, M.; Young, G.; Nagahama, Y.
    Isoleucine-15 of rainbow trout carbonyl reductase-like 20beta-hydroxysteroid dehydrogenase is critical for coenzyme (NADPH) binding (2000), Proc. Natl. Acad. Sci. USA, 97, 3079-3083.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Oncorhynchus mykiss

Protein Variants

Protein Variants Comment Organism
I15T in 20beta-HSD type A, cofactor binding is abolished Oncorhynchus mykiss
Q27K in 20beta-HSD type B, cofactor binding is reduced Oncorhynchus mykiss
T15I in 20beta-HSD type B, enzyme activity is restored Oncorhynchus mykiss

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
17alpha-hydroxyprogesterone + NADPH + H+ Oncorhynchus mykiss
-
17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ maturation-inducing hormone of oocytes ?

Organism

Organism UniProt Comment Textmining
Oncorhynchus mykiss
-
2 types of enzyme activity: carbonyl reductase + 20beta-hydroxysteroid dehydrogenase (type A) or only 20beta-hydroxysteroid dehydrogenase activity (type B)
-

Purification (Commentary)

Purification (Comment) Organism
recombinant from Escherichia coli Oncorhynchus mykiss

Reaction

Reaction Comment Organism Reaction ID
androstan-3alpha,17beta-diol + NAD+ = 17beta-hydroxyandrostan-3-one + NADH + H+ exhibits carbonyl reductase-like activity Oncorhynchus mykiss

Source Tissue

Source Tissue Comment Organism Textmining
ovarian follicle ovarian Oncorhynchus mykiss
-
ovary
-
Oncorhynchus mykiss
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.27
-
enzyme type B mutant Q27K, 17alpha-hydroxyprogesterone Oncorhynchus mykiss
1.57
-
enzyme type A, 17alpha-hydroxyprogesterone Oncorhynchus mykiss
1.64
-
enzyme type B mutant T15I, 17alpha-hydroxyprogesterone Oncorhynchus mykiss

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-hydroxyprogesterone + NADPH + H+ enzyme wild type A and B, mutants of enzyme type A and B Oncorhynchus mykiss 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+
-
?
17alpha-hydroxyprogesterone + NADPH + H+
-
Oncorhynchus mykiss 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ maturation-inducing hormone of oocytes ?