Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Oncorhynchus mykiss |
Protein Variants | Comment | Organism |
---|---|---|
I15T | in 20beta-HSD type A, cofactor binding is abolished | Oncorhynchus mykiss |
Q27K | in 20beta-HSD type B, cofactor binding is reduced | Oncorhynchus mykiss |
T15I | in 20beta-HSD type B, enzyme activity is restored | Oncorhynchus mykiss |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NADPH + H+ | Oncorhynchus mykiss | - |
17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ | maturation-inducing hormone of oocytes | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oncorhynchus mykiss | - |
2 types of enzyme activity: carbonyl reductase + 20beta-hydroxysteroid dehydrogenase (type A) or only 20beta-hydroxysteroid dehydrogenase activity (type B) | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli | Oncorhynchus mykiss |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
androstan-3alpha,17beta-diol + NAD+ = 17beta-hydroxyandrostan-3-one + NADH + H+ | exhibits carbonyl reductase-like activity | Oncorhynchus mykiss |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ovarian follicle | ovarian | Oncorhynchus mykiss | - |
ovary | - |
Oncorhynchus mykiss | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.27 | - |
enzyme type B mutant Q27K, 17alpha-hydroxyprogesterone | Oncorhynchus mykiss |
1.57 | - |
enzyme type A, 17alpha-hydroxyprogesterone | Oncorhynchus mykiss |
1.64 | - |
enzyme type B mutant T15I, 17alpha-hydroxyprogesterone | Oncorhynchus mykiss |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
17alpha-hydroxyprogesterone + NADPH + H+ | enzyme wild type A and B, mutants of enzyme type A and B | Oncorhynchus mykiss | 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+ | - |
? | |
17alpha-hydroxyprogesterone + NADPH + H+ | - |
Oncorhynchus mykiss | 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+ | maturation-inducing hormone of oocytes | ? |