Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.53 extracted from

  • Nakajin, S.; Ohno, S.; Shinoda, M.
    20beta-Hydroxysteroid dehydrogenase of neonatal pig testis: purification and some properties (1988), J. Biochem., 104, 565-569.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
Sus scrofa
cysteine slight Sus scrofa
dithiothreitol slight Sus scrofa
Hg2+
-
Sus scrofa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0015
-
progesterone
-
Sus scrofa
0.004
-
pregnenolone
-
Sus scrofa
0.0086
-
deoxycorticosterone
-
Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30500
-
gel filtration Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
17alpha-hydroxyprogesterone + NADPH + H+ Sus scrofa
-
17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+
-
?
progesterone + NADPH Sus scrofa
-
20beta-hydroxy-pregn-4-ene-3-one + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
testis
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.004
-
-
Sus scrofa

Storage Stability

Storage Stability Organism
-20°C, 50 mM potassium phosphate buffer, pH 7.4 Sus scrofa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
17alpha-hydroxypregnenolone + NADPH i.e. 3beta,17alpha-dihydroxypregn-5-ene-20-one Sus scrofa 3beta,17alpha,20beta-trihydroxypregn-5-ene + NADP+
-
?
17alpha-hydroxyprogesterone + NADPH + H+
-
Sus scrofa 17alpha,20beta-dihydroxy-pregn-4-ene-3-one + NADP+
-
?
17alpha-hydroxyprogesterone + NADPH + H+
-
Sus scrofa 17alpha,20beta-dihydroxy-4-pregnene-3-one + NADP+
-
?
deoxycorticosterone + NADPH i.e. 21-hydroxypregn-4-ene-3,20-dione, 21-hydroxyprogesterone Sus scrofa 20,21-dihydroxypregn-4-ene-3-one + NADP+
-
?
deoxycortisol + NADPH
-
Sus scrofa ?
-
?
pregnenolone + NADPH i.e. 3-hydroxypregn-5-en-20-one Sus scrofa 3beta,20beta-Dihydroxypregn-5-ene + NADP+
-
?
progesterone + NAD(P)H
-
Sus scrofa 20beta-hydroxy-pregn-4-ene-3-one + NAD(P)+
-
?
progesterone + NADPH
-
Sus scrofa 20beta-hydroxy-pregn-4-ene-3-one + NADP+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30500, SDS-PAGE with and without 2-mercaptoethanol Sus scrofa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Sus scrofa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 60 50% of maximal activity at 35°C, 10.4% of maximal activity at 60°C Sus scrofa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
up to Sus scrofa
55
-
inactivation at Sus scrofa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
reduction of oxosteroid Sus scrofa

pH Range

pH Minimum pH Maximum Comment Organism
4.5 5.5
-
Sus scrofa

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 10
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Sus scrofa