Application | Comment | Organism |
---|---|---|
synthesis | engineering Mycobacterium smegmatis for testosterone production. Mycobacterium smegmatis is an excellent chassis to develop biotechnological processes for the biotransformation of sterols and their derivatives into valuable pharmaceutical compounds. Overexpression of the gene encoding microbial 17beta-hydroxysteroid: NADP 17-oxidoreductase, from the bacterium Comamonas testosteroni. The host strains are Mycobacterium smegmatis wild type and a genetic engineered androst-4-ene-3,17-dione producing mutant. The recombinant strains are able to produce testosterone from androst-4-ene-3,17-dione and/or from sterols with high yields | Comamonas testosteroni |
synthesis | engineering Mycobacterium smegmatis for testosterone production. Mycobacterium smegmatis is an excellent chassis to develop biotechnological processes for the biotransformation of sterols and their derivatives into valuable pharmaceutical compounds. Overexpression of the gene encoding microbial 17beta-hydroxysteroid: NADP 17-oxidoreductase, from the fungus Cochliobolus lunatus. The host strains are Mycobacterium smegmatis wild type and a genetic engineered androst-4-ene-3,17-dione producing mutant. The recombinant strains are able to produce testosterone from androst-4-ene-3,17-dione and/or from sterols with high yields | Curvularia lunata |
Cloned (Comment) | Organism |
---|---|
- |
Curvularia lunata |
- |
Comamonas testosteroni |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
androst-4-ene-3,17-dione + NADPH + H+ | Curvularia lunata | - |
testosterone + NADP+ | - |
? | |
androst-4-ene-3,17-dione + NADPH + H+ | Comamonas testosteroni | - |
testosterone + NADP+ | - |
? | |
androst-4-ene-3,17-dione + NADPH + H+ | Comamonas testosteroni ATCC 11996 | - |
testosterone + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Comamonas testosteroni | P19871 | - |
- |
Comamonas testosteroni ATCC 11996 | P19871 | - |
- |
Curvularia lunata | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
androst-4-ene-3,17-dione + NADPH + H+ | - |
Curvularia lunata | testosterone + NADP+ | - |
? | |
androst-4-ene-3,17-dione + NADPH + H+ | - |
Comamonas testosteroni | testosterone + NADP+ | - |
? | |
androst-4-ene-3,17-dione + NADPH + H+ | - |
Comamonas testosteroni ATCC 11996 | testosterone + NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
17beta-HSD | - |
Curvularia lunata |
17beta-hydroxysteroid: NADP 17-oxidoreductase | - |
Curvularia lunata |
17beta-hydroxysteroid: NADP 17-oxidoreductase | - |
Comamonas testosteroni |
3beta/17beta-HSD | - |
Comamonas testosteroni |