Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pseudomonas sp. |
Crystallization (Comment) | Organism |
---|---|
purified recombinant enzyme in complex with NADH, hanging drop vapour diffusion method and microseeding, 0.002 ml of protein solution with 0.3 mM 3alpha-HSD and 0.4 mM NADH in 10 mM TrisHCl pH 8.0, are mixed with 0.002 ml reservoir solution optimally containing 0.1 M TrisHCl, pH 9.0, 0.14 M sodium chloride and 1.4 M ammonium sulfate, 4°C, X-ray diffraction structure determination and analysis at 1.8 A resolution | Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000, recombinant enzyme | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes the reversible interconversion of hydroxyl and oxo groups at position 3 of the steroid nucleus | Pseudomonas sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 26000, recombinant enzyme | Pseudomonas sp. |
Synonyms | Comment | Organism |
---|---|---|
3alpha-HSD | - |
Pseudomonas sp. |
3alpha-hydroxysteroid dehydrogenase | - |
Pseudomonas sp. |
More | the enzyme belongs to the short-chain dehydrogenase/reductase, SDR, superfamily | Pseudomonas sp. |
NAD(P)+-3alpha-hydroxysteroid dehydrogenase | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Pseudomonas sp. |