KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | steady-state kinetics | Pseudomonas sp. | |
0.0062 | - |
Fusidic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
0.031 | - |
cholic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
0.037 | - |
Fusidic acid | pH 8.5, 37°C, with cofactor thio-NAD+ | Pseudomonas sp. | |
0.038 | - |
glycocholic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
0.043 | - |
taurocholic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
0.063 | - |
chenodeoxycholic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
0.068 | - |
lithocholic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
0.069 | - |
(3alpha,5beta,7beta)-3,7-dihydroxycholan-24-oic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
0.084 | - |
cholic acid | pH 8.5, 37°C, with cofactor thio-NAD+ | Pseudomonas sp. | |
0.21 | - |
androsterone | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
srain B-0831 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
androsterone + NAD+ | - |
Pseudomonas sp. | 5alpha-androstane-3,17-dione + NADH | - |
? | |
chenodeoxycholic acid + NAD+ | - |
Pseudomonas sp. | 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oic acid + NADH | - |
? | |
cholic acid + NAD+ | - |
Pseudomonas sp. | 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oic acid + NADH | - |
? | |
cholic acid + thio-NAD+ | - |
Pseudomonas sp. | 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oic acid + thio-NADH | - |
? | |
fusidic acid + NAD+ | antibiotic steroid substrate | Pseudomonas sp. | 3-oxofusidic acid + NADH | - |
? | |
glycocholic acid + NAD+ | - |
Pseudomonas sp. | 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oyl-glycine + NADH | - |
? | |
lithocholic acid + NAD+ | - |
Pseudomonas sp. | 5beta-cholan-3-one-24-oic acid + NADH | - |
? | |
additional information | broad steroid substrate and cofactor specificity, no 3-oxosteroid reduction of testosterone | Pseudomonas sp. | ? | - |
? | |
taurocholic acid + NAD+ | - |
Pseudomonas sp. | 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oyl-taurine + NADH | - |
? | |
ursodeoxycholic acid + NAD+ | - |
Pseudomonas sp. | 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oic acid + NADH | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3alpha-hydroxysteroid dehydrogenase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
23 | - |
Fusidic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
25 | - |
Fusidic acid | pH 8.5, 37°C, with cofactor thio-NAD+ | Pseudomonas sp. | |
35 | - |
cholic acid | pH 8.5, 37°C, with cofactor thio-NAD+ | Pseudomonas sp. | |
72 | - |
lithocholic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
74 | - |
taurocholic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
75 | - |
cholic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
76 | - |
chenodeoxycholic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
77 | - |
glycocholic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
82 | - |
(3alpha,5beta,7beta)-3,7-dihydroxycholan-24-oic acid | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. | |
134 | - |
androsterone | pH 8.5, 37°C, with cofactor NAD+ | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine adenine dinucleotide | i.e. APAD+, 42% of the activity with NAD+ when cholic acid is utilized as substrate | Pseudomonas sp. | |
additional information | cofactor specificity with different substrates | Pseudomonas sp. | |
NAD+ | best cofactor, preferably utilized with substrate cholic acid | Pseudomonas sp. | |
nicotinamide hypoxanthine dinucleotide | i.e. deamino-NAD+, 36% of the activity with NAD+ when cholic acid is utilized as substrate | Pseudomonas sp. | |
thio-NAD+ | 47% of the activity with NAD+ when cholic acid is utilized as substrate, preferably utilized with substrate fusidic acid | Pseudomonas sp. |