Crystallization (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
General Stability | Organism |
---|---|
stable in solution for a long period | Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
NAD+ | - |
Pseudomonas putida | |
0.0136 | - |
deoxycholate | - |
Pseudomonas putida | |
0.015 | - |
cholate | - |
Pseudomonas putida |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Pseudomonas putida | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
non-denaturing gel electrophoresis and gel filtration | Pseudomonas putida |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas putida | belongs to the group of Y'bile acid binders, bile acid metabolizing enzymes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas putida | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas putida |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
23.6 | - |
purified enzyme, substrate cholate with NAD+ | Pseudomonas putida |
Storage Stability | Organism |
---|---|
-80°C, lyophilized or ammonium sulfate precipitated, more than 1 year | Pseudomonas putida |
4°C, diluted in Tris-HCl, pH 8.0, with ß-mercaptoethanol, 20 days | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl glycine + NAD+ | - |
Pseudomonas putida | 7alpha,12alpha-dihydroxy-5beta-cholan-3-one-24-oyl glycine + NADH | - |
? | |
6-ketolithocholate + NAD+ | - |
Pseudomonas putida | 5beta-cholan-3,6-dione-24-oic acid + NADH | - |
r | |
androsterone + NAD(P)+ | - |
Pseudomonas putida | 5alpha-androstan-3,17-dione + NAD(P)H | - |
? | |
chenodeoxycholic acid + NAD(P)+ | - |
Pseudomonas putida | 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
r | |
cholic acid + NAD(P)+ | - |
Pseudomonas putida | 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H | - |
? | |
deoxycholic acid + NAD(P)+ | - |
Pseudomonas putida | 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+ | - |
? | |
additional information | belongs to the group of Y'bile acid binders, bile acid metabolizing enzymes | Pseudomonas putida | ? | - |
? | |
taurocholic acid + NAD(P)+ | - |
Pseudomonas putida | 2[[7alpha,12alpha-dihydroxy-3,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADPH | - |
? | |
ursodeoxycholate + NAD+ | - |
Pseudomonas putida | 7beta-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 25000, SDS-PAGE | Pseudomonas putida |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
heating for 10 min, rapid heat denaturation over 50°C | Pseudomonas putida |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | 11 | oxidation of cholate | Pseudomonas putida |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | - |
Pseudomonas putida |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific, enzyme does not use NADP+ | Pseudomonas putida |