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Literature summary for 1.1.1.50 extracted from

  • Uwajima, T.; Terada, O.
    Properties of crystalline 3alpha-hydroxysteroid dehydrogenase from Pseudomonas putida (1979), Agric. Biol. Chem., 43, 1521-1528.
No PubMed abstract available
Search for more literature for 1.1.1.50

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Pseudomonas putida

General Stability

General Stability Organism
mercaptoethanol stabilizes Pseudomonas putida

Inhibitors

Inhibitors Comment Organism Structure
heavy metal ions
-
 Pseudomonas putida
p-chloromercuribenzoate
-
 Pseudomonas putida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01 0.025 cholic acid
-
 Pseudomonas putida
0.87
-
 NAD+
-
 Pseudomonas putida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 sedimentation equilibrium centrifugation Pseudomonas putida

Organism

Organism UniProt Comment Textmining
Pseudomonas putida
-
-
-

Storage Stability

Storage Stability Organism
0-3°C, lyophilized, at least 6 months Pseudomonas putida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3alpha-hydroxy-12-oxo-9,11-cholenic acid + NADP+
-
 Pseudomonas putida 3,12-dioxo-9,11-cholenic acid + NADPH
-
 ?
5alpha-dihydrotestosterone + NAD(P)H + H+ i.e. 5alpha-androstan-17beta-ol-3-one Pseudomonas putida 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
 r
5alpha-dihydrotestosterone + NAD(P)H + H+ i.e. 5alpha-androstan-17beta-ol-3-one Pseudomonas putida (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
 r
androsterone + NAD(P)+ i.e. 3alpha-hydroxy-5alpha-androstan-17-one Pseudomonas putida 5alpha-androstan-3,17-dione + NAD(P)H
-
 r
chenodeoxycholic acid + NAD(P)+ i.e. 3alpha,7alpha-dihydroxy-5beta-cholan-24-oic acid Pseudomonas putida 7alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 r
cholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oic acid Pseudomonas putida 7alpha,12alpha-dihydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H
-
 ?
deoxycholic acid + NAD(P)+ i.e. 3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid Pseudomonas putida 12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NAD(P)H + H+
-
 ?
etiocholan-3alpha-ol-17-one + NADP+
-
 Pseudomonas putida etiocholan-3,17-dione + NADPH
-
 ?
lithocholic acid + NADP+ i.e. 3alpha-hydroxy-5beta-cholan-24-oic acid Pseudomonas putida 3-oxo-5beta-cholan-24-oic acid + NADPH
-
 ?
additional information acts faster on A/B cis hydroxysteroids than on A/B trans hydroxysteroids Pseudomonas putida ?
-
 ?
additional information absolutely specific for 3alpha-hydroxy group of C19, C21 and C24 steroids Pseudomonas putida ?
-
 ?
taurocholic acid + NAD(P)+ i.e. 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanoyl taurine Pseudomonas putida 2[[7alpha,12alpha-dihydroxy-3,24-dioxo-5beta-cholan-24-yl]amino]ethane sulfonic acid + NADPH
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, sedimentation equilibrium, presence of 2-mercaptoethanol Pseudomonas putida

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 15 min stable in presence of mercaptoethanol, 10% loss of activity in absence of mercaptoethanol Pseudomonas putida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
 reduction of etiocholan-3,17-dione Pseudomonas putida

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10.5 stable for 30 min at 30°C Pseudomonas putida

Cofactor

Cofactor Comment Organism Structure
additional information see also EC 1.1.1.213 for enzymes catalyzing the same reaction but with A-specificity concerning the cofactor, all enzymes without information of stereospecificity for cofactors are included in EC 1.1.1.50, but some of those may in fact belong to EC 1.1.1.213 Pseudomonas putida