Literature summary for 1.1.1.47 extracted from

Liu, X.; Du, X.; Feng, J.; Wu, M.; Lin, J.; Guan, J.; Wang, T.; Zhang, Z.
Co-immobilization of short-chain dehydrogenase/reductase and glucose dehydrogenase for the efficient production of (�)-ethyl mandelate (2019), Catal. Lett., 149, 1710-1720 .

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Application

Application	Comment	Organism
biotechnology	(±)-ethyl mandelate are important intermediates in the synthesis of numerous pharmaceuticals. Efficient routes for the production of these derivatives are highly desirable. A co-immobilization strategy is developed to overcome the issue of NADPH demand in the short-chain dehydrogenase/reductase (SDR) catalytic process. The SDR from Thermus thermophilus HB8 and the NAD(P)-dependent glucose dehydrogenase (GDH) from Thermoplasma acidophilum DSM 1728 are co-immobilized on silica gel. This dual-system offers an efficient route for the biosynthesis of (+/-)-ethyl mandelate	Thermoplasma acidophilum
synthesis	(±)-ethyl mandelate are important intermediates in the synthesis of numerous pharmaceuticals. Efficient routes for the production of these derivatives are highly desirable. A co-immobilization strategy is developed to overcome the issue of NADPH demand in the short-chain dehydrogenase/reductase (SDR) catalytic process. The SDR from Thermus thermophilus HB8 and the NAD(P)-dependent glucose dehydrogenase (GDH) from Thermoplasma acidophilum DSM 1728 are co-immobilized on silica gel. This dual-system offers an efficient route for the biosynthesis of (+/-)-ethyl mandelate	Thermoplasma acidophilum

Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli, short-chain dehydrogenase/reductase from Thermus thermophilus HB8 and the glucose dehydrogenase from Thermoplasma acidophilum DSM 1728 are simultaneously over-expressed by heterologous expression with pETDuet	Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
40250	-	calculated from sequence	Thermoplasma acidophilum

Organism

Organism	UniProt	Comment	Textmining
Thermoplasma acidophilum	-	-	-

Synonyms

Synonyms	Comment	Organism
NAD(P)-dependent glucose dehydrogenase	-	Thermoplasma acidophilum

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Release 2023.1 (January 2023)