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Literature summary for 1.1.1.47 extracted from

  • Shatton, J.B.; Halver, J.E.; Weinhouse, S.
    Glucose (hexose 6-phosphate) dehydrogenase in liver of rainbow trout (1971), J. Biol. Chem., 246, 4878-4885.
    View publication on PubMed
Search for more literature for 1.1.1.47

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
 galactose 6-phosphate pH 7.5, 25°C, coenzyme NADP+ Oncorhynchus mykiss
0.01
-
 D-glucose 6-phosphate pH 7.5, 25°C, coenzyme NAD+ Oncorhynchus mykiss
0.01
-
 galactose 6-phosphate at pH 7.5 and 25°C Oncorhynchus mykiss
0.02
-
 D-glucose 6-phosphate pH 7.5, 25°C, coenzyme NADP+ Oncorhynchus mykiss
0.04
-
 D-galactose 6-phosphate pH 7.5, 25°C, coenzyme NAD+ Oncorhynchus mykiss
0.04
-
 D-galactose 6-phosphate at pH 7.5 and 25°C Oncorhynchus mykiss
0.05
-
 NAD+ pH 7.5, 25°C Oncorhynchus mykiss
0.09
-
 NAD+ pH 10, 25°C Oncorhynchus mykiss
0.09
-
 NADP+ pH 10, 25°C Oncorhynchus mykiss
0.1
-
 NADP+ pH 7.5, 25°C Oncorhynchus mykiss
0.1
-
 D-glucose 6-phosphate pH 10, 25°C, coenzyme NAD+ or NADP+ Oncorhynchus mykiss
0.3
-
 2-deoxy-D-glucose 6-phosphate pH 7.5, 25°C, coenzyme NAD+ Oncorhynchus mykiss
1
-
 2-deoxy-D-glucose 6-phosphate pH 7.5, 25°C, coenzyme NADP+ Oncorhynchus mykiss
3
-
 D-galactose 6-phosphate pH 10, 25°C, coenzyme NAD+ Oncorhynchus mykiss
3
-
 D-galactose 6-phosphate at pH 10.0 and 25°C Oncorhynchus mykiss
5
-
 D-galactose 6-phosphate pH 10, 25°C, coenzyme NADP+ Oncorhynchus mykiss
5
-
 D-galactose 6-phosphate at pH 10.0 and 25°C Oncorhynchus mykiss
20
-
 beta-D-glucose pH 10, 25°C, coenzyme NAD+ Oncorhynchus mykiss
25
-
 2-deoxy-D-glucose 6-phosphate pH 10, 25°C, coenzyme NADP+ Oncorhynchus mykiss
40
-
 beta-D-glucose pH 10, 25°C, coenzyme NADP+ Oncorhynchus mykiss
70
-
 D-xylose pH 10, 25°C, coenzyme NAD+ Oncorhynchus mykiss
80
-
 D-glucose pH 7.5, 25°C, coenzyme NADP+ Oncorhynchus mykiss
80
-
 D-xylose pH 7.5, 25°C, coenzyme NAD+ Oncorhynchus mykiss
80
-
 D-glucose at pH 7.5 and 25°C Oncorhynchus mykiss
100
-
 D-xylose pH 7.5, 25°C, coenzyme NADP+ Oncorhynchus mykiss
200
-
 D-xylose pH 7.5, 25°C, coenzyme NADP+ Oncorhynchus mykiss
800
-
 D-galactose about, pH 7.5, 25°C, coenzyme NAD+ Oncorhynchus mykiss
1000
-
 D-galactose about, pH 10, 25°C, coenzyme NAD+ Oncorhynchus mykiss
4000
-
 D-galactose about, pH 10, 25°C, coenzyme NADP+ Oncorhynchus mykiss
10000
-
 D-galactose pH 7.5, 25°C, coenzyme NADP+ Oncorhynchus mykiss

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ without Mg2+ low activity with coenzyme NADP Oncorhynchus mykiss

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
180000
-
 gel filtration Oncorhynchus mykiss

Organism

Organism UniProt Comment Textmining
Oncorhynchus mykiss
-
-
-

Purification (Commentary)

Purification (Comment) Organism
calcium-phosphate gel, ammonium sulfate, DEAE-cellulose Oncorhynchus mykiss

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.6
-
-
 Oncorhynchus mykiss

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-deoxy-D-glucose 6-phosphate + NAD+
-
 Oncorhynchus mykiss 2-deoxy-D-glucono-1,5-lactone 6-phosphate + NADH
-
 ?
2-deoxy-D-glucose 6-phosphate + NADP+
-
 Oncorhynchus mykiss 2-deoxy-D-glucono-1,5-lactone 6-phosphate + NADPH
-
 ?
beta-D-glucose + NAD+
-
 Oncorhynchus mykiss D-glucono-1,5-lactone + NADH + H+
-
 ?
beta-D-glucose + NADP+
-
 Oncorhynchus mykiss D-glucono-1,5-lactone + NADPH + H+
-
 ?
beta-D-glucose 6-phosphate + NAD+
-
 Oncorhynchus mykiss D-glucono-1,5-lactone 6-phosphate + NADPH
-
 ?
beta-D-glucose 6-phosphate + NADP+
-
 Oncorhynchus mykiss D-glucono-1,5-lactone 6-phosphate + NADPH
-
 ?
D-galactose + NAD+
-
 Oncorhynchus mykiss D-galactono-1,5-lactone + NADH
-
 ?
D-galactose + NADP+
-
 Oncorhynchus mykiss D-galactono-1,5-lactone + NADPH
-
 ?
D-galactose 6-phosphate + NAD+
-
 Oncorhynchus mykiss 6-phospho-D-galactono-1,5-lactone + NADH + H+
-
 r
D-galactose 6-phosphate + NADP+
-
 Oncorhynchus mykiss 6-phospho-D-galactono-1,5-lactone + NADPH + H+
-
 r
D-glucose + NADP+
-
 Oncorhynchus mykiss D-glucono-1,5-lactone + NADPH + H+
-
 r
D-glucose 6-phosphate + NADP+
-
 Oncorhynchus mykiss D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?
D-xylose + NAD+
-
 Oncorhynchus mykiss D-xylono-1,5-lactone + NADH + H+
-
 ?
D-xylose + NADP+
-
 Oncorhynchus mykiss D-xylono-1,5-lactone + NADPH + H+
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 5 min, 40% loss of activity Oncorhynchus mykiss
50
-
 5 min, complete inactivation Oncorhynchus mykiss

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10.5
-
 activity with D-glucose 6-phosphate and NADP+ Oncorhynchus mykiss

pH Range

pH Minimum pH Maximum Comment Organism
8.5 12 pH 8.5: about 35% of maximal activity, pH 12.0: about 65% of maximal activity Oncorhynchus mykiss

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Oncorhynchus mykiss
NADP+
-
 Oncorhynchus mykiss