Literature summary for 1.1.1.47 extracted from

Smith, L.D.; Budgen, N.; Bungard, S.J.; Danson, M.J.; Hough, D.W.
Purification and characterization of glucose dehydrogenase from the thermoacidophilic archaebacterium Thermoplasma acidophilum (1989), Biochem. J., 261, 973-977.

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General Stability

General Stability	Organism
4 M urea, room temperature, 6 h, 100% activity	Thermoplasma acidophilum

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.113	-	NADP+	pH 7.0, 55°C	Thermoplasma acidophilum
10	-	beta-D-glucose	pH 7.0, 55°C	Thermoplasma acidophilum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	4 * 38000, SDS-PAGE	Thermoplasma acidophilum
155000	-	gel filtration	Thermoplasma acidophilum

Organic Solvent Stability

Organic Solvent	Comment	Organism
Acetone	50% v/v, room temperature, 6 h, stable	Thermoplasma acidophilum
Ethanol	50% v/v, room temperature, 6 h, stable	Thermoplasma acidophilum
Methanol	50% v/v, room temperature, 6 h, stable	Thermoplasma acidophilum

Organism

Organism	UniProt	Comment	Textmining
Thermoplasma acidophilum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
methanol extraction, Mono Q, Matrex Gel Red A, Mono P, gel filtration	Thermoplasma acidophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
320	-	-	Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-glucose + NAD+	-	Thermoplasma acidophilum	D-glucono-1,5-lactone + NADH + H+	-	?
beta-D-glucose + NADP+	-	Thermoplasma acidophilum	D-glucono-1,5-lactone + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
tetramer	4 * 38000, SDS-PAGE	Thermoplasma acidophilum

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	-	9 h, stable	Thermoplasma acidophilum
75	-	half-life: approximately 3 h	Thermoplasma acidophilum

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Thermoplasma acidophilum
NADP+	-	Thermoplasma acidophilum

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Release 2023.1 (January 2023)