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Literature summary for 1.1.1.44 extracted from

  • Chan, B.; Sukhatme, V.P.
    One-step purification of soluble recombinant human 6-phosphogluconate dehydrogenase from Escherichia coli (2013), Protein Expr. Purif., 92, 62-66.
    View publication on PubMed
Search for more literature for 1.1.1.44

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta2 (DE3) cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3-phosphoglycerate about 40% residual activity at 5 mM Homo sapiens
additional information not inhibited by fumarate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 NADP+ at pH 7.5 and 22°C Homo sapiens
0.051
-
 6-phospho-D-gluconate at pH 7.5 and 22°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NADP+ Homo sapiens
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P52209
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
endothelial cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
 Homo sapiens D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 ?

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
 Homo sapiens
6PGD
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
71
-
 NADP+ at pH 7.5 and 22°C Homo sapiens
73
-
 6-phospho-D-gluconate at pH 7.5 and 22°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Homo sapiens