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Literature summary for 1.1.1.44 extracted from

  • Zera, A.J.; Wehrkamp, C.; Schilder, R.; Black, C.; Gribben, P.
    Purification and characterization of 6-phosphogluconate dehydrogenase from the wing-polymorphic cricket, Gryllus firmus, and assessment of causes of morph-differences in enzyme activity (2014), Comp. Biochem. Physiol. B, 172-173, 29-38.
    View publication on PubMed

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Gryllus firmus 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49000
-
2 * 49000, SDS-PAGE Gryllus firmus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NADP+ Gryllus firmus
-
D-ribulose 5-phosphate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Gryllus firmus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
PEG precipitation, Cibacron Blue column chromatography, ADP agarose column chromatography, and phenyl Sepharose column chromatography Gryllus firmus

Source Tissue

Source Tissue Comment Organism Textmining
fat body
-
Gryllus firmus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0169
-
unpurified enzyme, at pH 8.0 and 28°C Gryllus firmus
13.5
-
after 799fold purification, at pH 8.0 and 28°C Gryllus firmus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
Gryllus firmus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 49000, SDS-PAGE Gryllus firmus

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
Gryllus firmus
6PGDH
-
Gryllus firmus

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Gryllus firmus