Inhibitors | Comment | Organism | Structure |
---|---|---|---|
NADPH | complete inhibition | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.053 | - |
NADP+ | - |
Rattus norvegicus | |
0.595 | - |
6-phospho-D-gluconate | - |
Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
2 * 52000, SDS-PAGE | Rattus norvegicus |
104000 | - |
SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephadex G-25 column chromatography, DEAE-Sepharose Fast Flow ion exchange chromatography and 2',5'-ADP Sepharose 4B column chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
small intestine | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.032 | - |
supernantant | Rattus norvegicus |
15.12 | - |
after 473fold purification | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NADP+ | - |
Rattus norvegicus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 52000, SDS-PAGE | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
6-GPD | - |
Rattus norvegicus |
6-phosphogluconate dehydrogenase | - |
Rattus norvegicus |
6-phosphogluconate:NADP oxidoreductase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
49.4 | - |
- |
Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
7 | 9 | with increasing phosphate buffer concentration from 25 to 150 mM | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.35 | - |
- |
Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03191 | - |
NADPH | competitive inhibitor | Rattus norvegicus |