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Literature summary for 1.1.1.44 extracted from
- Purification and kinetic properties of 6-phosphogluconate dehydrogenase from rat small intestine (2005), Protein J., 24, 293-301.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | complete inhibition | Rattus norvegicus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.053 | - |
NADP+ | - |
Rattus norvegicus | |
| 0.595 | - |
6-phospho-D-gluconate | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 52000 | - |
2 * 52000, SDS-PAGE | Rattus norvegicus |
| 104000 | - |
SDS-PAGE | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Sephadex G-25 column chromatography, DEAE-Sepharose Fast Flow ion exchange chromatography and 2',5'-ADP Sepharose 4B column chromatography | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| small intestine | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.032 | - |
supernantant | Rattus norvegicus |
| 15.12 | - |
after 473fold purification | Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-phospho-D-gluconate + NADP+ | - |
Rattus norvegicus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 52000, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 6-GPD | - |
Rattus norvegicus |
| 6-phosphogluconate dehydrogenase | - |
Rattus norvegicus |
| 6-phosphogluconate:NADP oxidoreductase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 49.4 | - |
- |
Rattus norvegicus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 7 | 9 | with increasing phosphate buffer concentration from 25 to 150 mM | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.35 | - |
- |
Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Rattus norvegicus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03191 | - |
NADPH | competitive inhibitor | Rattus norvegicus | |
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Release 2023.1 (January 2023)
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