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Literature summary for 1.1.1.44 extracted from

  • Li, L.; Zhang, L.; Cook, P.F.
    Role of the S128, H186, and N187 triad in substrate binding and decarboxylation in the sheep liver 6-phosphogluconate dehydrogenase reaction (2006), Biochemistry, 45, 12680-12686.
    View publication on PubMed
Search for more literature for 1.1.1.44

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain M15[pREP4] Ovis aries

Protein Variants

Protein Variants Comment Organism
H186A reduced activity compared to the wild type enzyme Ovis aries
H187A reduced activity compared to the wild type enzyme Ovis aries
S128A reduced activity compared to the wild type enzyme Ovis aries

Inhibitors

Inhibitors Comment Organism Structure
NADPH saturating concentration at pH 7.5 Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 NADP+ mutant enzyme H186A Ovis aries
0.005
-
 NADP+ wild type enzyme Ovis aries
0.0054
-
 NADP+ mutant enzyme S128A Ovis aries
0.0121
-
 6-phospho-D-gluconate mutant enzyme H186A Ovis aries
0.0284
-
 6-phospho-D-gluconate wild type enzyme Ovis aries
0.0298
-
 NADP+ mutant enzyme N187A Ovis aries
0.095
-
 6-phospho-D-gluconate mutant enzyme S128A Ovis aries
0.53
-
 6-phospho-D-gluconate mutant enzyme N187A Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries P00349
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
 Ovis aries
6PGDH
-
 Ovis aries

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Ovis aries

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.008
-
 NADPH wild type enzyme, at a saturating concentration of NADPH at pH 7.5 Ovis aries
0.0093
-
 NADPH mutant enzyme N187A, at a saturating concentration of NADPH at pH 7.5 Ovis aries
0.019
-
 NADPH mutant enzyme S128A, at a saturating concentration of NADPH at pH 7.5 Ovis aries
0.027
-
 NADPH mutant enzyme H186A, at a saturating concentration of NADPH at pH 7.5 Ovis aries