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Literature summary for 1.1.1.44 extracted from

  • Cervellati, C.; Dallocchio, F.; Bergamini, C.M.; Cook, P.F.
    Role of methionine-13 in the catalytic mechanism of 6-phosphogluconate dehydrogenase from sheep liver (2005), Biochemistry, 44, 2432-2440.
    View publication on PubMed
Search for more literature for 1.1.1.44

Protein Variants

Protein Variants Comment Organism
M13C mutant showing decrease in affinity for NADP+, but not NADPH Ovis aries
M13F mutant showing decrease in affinity for NADP+, but not NADPH Ovis aries
M13I mutant showing decrease in affinity for NADP+, but not NADPH Ovis aries
M13Q mutant showing decrease in affinity for NADP+, but not NADPH Ovis aries
M13V mutant showing decrease in affinity for NADP+, but not NADPH Ovis aries

Inhibitors

Inhibitors Comment Organism Structure
NADPH competitive inhibition versus NADP+ with 6-phospho-D-gluconate equal to its Km and at a saturating concentration Ovis aries

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 NADP+ wild type enzyme Ovis aries
0.0064
-
 NADP+ mutant enzyme M13C Ovis aries
0.019
-
 NADP+ mutant enzyme M13F Ovis aries
0.019
-
 NADP+ mutant enzyme M13Q Ovis aries
0.026
-
 NADP+ mutant enzyme M13V Ovis aries
0.032
-
 NADP+ mutant enzyme M13I Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries P00349
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA resin column chromatography and 2?5?ADP agarose chromatography Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Ovis aries
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
6-phosphogluconate dehydrogenase
-
 Ovis aries
6PGDH
-
 Ovis aries

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Ovis aries

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00065
-
 NADPH mutant enzyme M13C, at a saturating concentration Ovis aries
0.001
-
 NADPH mutant enzyme M13I, at a saturating concentration Ovis aries
0.0011
-
 NADPH wild type enzyme, at a saturating concentration Ovis aries
0.0015
-
 NADPH mutant enzyme M13F, at a saturating concentration Ovis aries
0.0021
-
 NADPH mutant enzyme M13Q, at a saturating concentration Ovis aries
0.006
-
 NADPH mutant enzyme M13V, at a saturating concentration Ovis aries