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Literature summary for 1.1.1.44 extracted from
- Purification and properties of an NADP-specific 6-phosphogluconate dehydrogenase from Streptococcus faecalis (1975), J. Biol. Chem., 250, 6093-6100.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Cyberlindnera jadinii |  |
| 5,5'-dithiobis(2-nitrobenzoic acid) | - |
Enterococcus faecalis | |
| D-fructose 1,6-diphosphate | - |
Enterococcus faecalis | |
| D-fructose 1,6-diphosphate | - |
Neurospora crassa | |
| p-hydroxymercuribenzoate | - |
Enterococcus faecalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
6-phosphogluconate | - |
Neurospora crassa | |
| 0.013 | - |
NADP+ | - |
Rattus norvegicus | |
| 0.015 | - |
NADP+ | - |
Neurospora crassa | |
| 0.015 | - |
NADP+ | - |
Enterococcus faecalis | |
| 0.02 | - |
6-phosphogluconate | - |
Homo sapiens | |
| 0.02 | - |
NADP+ | type I and II | Cyberlindnera jadinii | |
| 0.024 | - |
6-phosphogluconate | - |
Enterococcus faecalis | |
| 0.03 | - |
NADP+ | - |
Homo sapiens | |
| 0.03 | - |
NADP+ | - |
Neurospora crassa | |
| 0.031 | - |
6-phosphogluconate | type II | Cyberlindnera jadinii | |
| 0.052 | - |
6-phosphogluconate | type I | Cyberlindnera jadinii | |
| 0.071 | - |
6-phosphogluconate | - |
Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
2 * 50000, type I, SDS-PAGE | Cyberlindnera jadinii |
| 52000 | - |
2 * 52000, SDS-PAGE | Homo sapiens |
| 52000 | - |
2 * 52000, SDS-PAGE | Rattus norvegicus |
| 52000 | - |
2 * 52000, SDS-PAGE | Enterococcus faecalis |
| 57000 | - |
2 * 57000, SDS-PAGE | Neurospora crassa |
| 101000 | - |
type I, centrifugation in sucrose density gradient | Cyberlindnera jadinii |
| 102000 | - |
- |
Rattus norvegicus |
| 104000 | - |
- |
Homo sapiens |
| 106000 | 115000 | gel filtration | Enterococcus faecalis |
| 108000 | - |
sedimentation equilibrium | Enterococcus faecalis |
| 110000 | 120000 | - |
Neurospora crassa |
| 111000 | - |
type II | Cyberlindnera jadinii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-phospho-D-gluconate + NADP+ | Cyberlindnera jadinii | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | Homo sapiens | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | Rattus norvegicus | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | Neurospora crassa | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | Enterococcus faecalis | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera jadinii | - |
type I, type II | - |
| Enterococcus faecalis | - |
ATCC 27792, formerly designated as strain MR | - |
| Homo sapiens | - |
human | - |
| Homo sapiens | - |
Krebs ascites cells | - |
| Neurospora crassa | - |
- |
- |
| Rattus norvegicus | - |
rat | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Cyberlindnera jadinii |
- |
Homo sapiens |
- |
Rattus norvegicus |
- |
Neurospora crassa |
- |
Enterococcus faecalis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
| liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.47 | - |
- |
Enterococcus faecalis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, purified enzyme is stable to storage in 50 mM Tris HCl buffer, pH 7.5, 10 mM dithiothreitol and 20% glycerol, no significant decrease in activity occurs over a 2 week period | Enterococcus faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-phospho-D-gluconate + NADP+ | - |
Cyberlindnera jadinii | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | - |
Homo sapiens | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | - |
Rattus norvegicus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | - |
Neurospora crassa | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| 6-phospho-D-gluconate + NADP+ | - |
Enterococcus faecalis | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
| D-ribulose 5-phosphate + CO2 + NADPH | reverse reaction only to a very limited extent | Enterococcus faecalis | 6-phospho-D-gluconate + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 57000, SDS-PAGE | Neurospora crassa |
| dimer | 2 * 52000, SDS-PAGE | Homo sapiens |
| dimer | 2 * 52000, SDS-PAGE | Rattus norvegicus |
| dimer | 2 * 52000, SDS-PAGE | Enterococcus faecalis |
| dimer | 2 * 50000, type I, SDS-PAGE | Cyberlindnera jadinii |
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