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Literature summary for 1.1.1.44 extracted from
- 6-Phosphogluconate dehydrogenase from Streptococcus faecalis (1975), Methods Enzymol., 41, 232-237.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | - |
Enterococcus faecalis |  |
| Cu2+ | - |
Enterococcus faecalis | |
| D-fructose 1,6-diphosphate | - |
Enterococcus faecalis | |
| p-chloromercuribenzoate | - |
Enterococcus faecalis | |
| Zn2+ | - |
Enterococcus faecalis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
NADP+ | - |
Enterococcus faecalis | |
| 0.024 | - |
6-phosphogluconate | - |
Enterococcus faecalis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 108000 | - |
sedimentation equilibrium | Enterococcus faecalis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-phospho-D-gluconate + NADP+ | Enterococcus faecalis | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterococcus faecalis | - |
ATCC 27792, formerly designated as strain MR | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Enterococcus faecalis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 14.47 | - |
- |
Enterococcus faecalis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 5°C, purified enzyme is stable to storage in 50 mM Tris HCl buffer, pH 7.5, 10 mM dithiothreitol and 20% glycerol, no significant decrease in activity occurs over a 2 week period | Enterococcus faecalis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 6-phospho-D-gluconate + NADP+ | - |
Enterococcus faecalis | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | SDS-PAGE | Enterococcus faecalis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-gluconate-6-phosphate dehydrogenase | - |
Enterococcus faecalis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | 8 | - |
Enterococcus faecalis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | specific for NADP+, no activity observed when NAD+ is substituted for NADP+ | Enterococcus faecalis | |
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