Application | Comment | Organism |
---|---|---|
synthesis | thermostability may lead to some practical applications | Geobacillus stearothermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoic acid) | - |
Cyberlindnera jadinii | |
5,5'-dithiobis(2-nitrobenzoic acid) | Ellman's reagent | Geobacillus stearothermophilus | |
Hg2+ | - |
Geobacillus stearothermophilus | |
p-chloromercuribenzoate | - |
Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
NADP+ | 60°C | Geobacillus stearothermophilus | |
0.014 | - |
6-phosphogluconate | 60°C or 65°C | Geobacillus stearothermophilus | |
0.015 | - |
NADP+ | 65°C | Geobacillus stearothermophilus | |
0.016 | - |
6-phosphogluconate | 30°C | Geobacillus stearothermophilus | |
0.017 | - |
NADP+ | 30°C | Geobacillus stearothermophilus | |
0.02 | - |
6-phosphogluconate | 43°C | Geobacillus stearothermophilus | |
0.021 | - |
NADP+ | 45°C | Geobacillus stearothermophilus | |
0.022 | - |
6-phosphogluconate | 45°C | Geobacillus stearothermophilus | |
0.025 | - |
NADP+ | 43°C | Geobacillus stearothermophilus | |
0.55 | - |
2-deoxy-6-phosphogluconate | - |
Cyberlindnera jadinii | |
1 | - |
NAD+ | 43°C | Cyberlindnera jadinii | |
3.5 | - |
NAD+ | 30°C | Cyberlindnera jadinii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | activates the enzyme at high concentrations | Geobacillus stearothermophilus | |
Mg2+ | - |
Cyberlindnera jadinii | |
Mg2+ | activates the enzyme at low concentrations 0.01-0.04 M | Geobacillus stearothermophilus | |
NaCl | activates the enzyme at high concentrations | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
2 * 51000, SDS-PAGE | Geobacillus stearothermophilus |
101000 | - |
- |
Cyberlindnera jadinii |
101000 | - |
sucrose density gradient centrifugation | Geobacillus stearothermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NADP+ | Cyberlindnera jadinii | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Homo sapiens | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Rattus norvegicus | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Geobacillus stearothermophilus | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Neurospora crassa | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | Geobacillus stearothermophilus NCA 1503 | - |
D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cyberlindnera jadinii | - |
- |
- |
Geobacillus stearothermophilus | - |
NCA 1503 | - |
Geobacillus stearothermophilus NCA 1503 | - |
NCA 1503 | - |
Homo sapiens | - |
human | - |
Neurospora crassa | - |
- |
- |
Rattus norvegicus | - |
rat | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
87 | - |
- |
Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
4°C, stable for several days, as an (NH4)2SO4 suspension stable for several months without any loss of enzyme activity | Cyberlindnera jadinii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-phospho-D-gluconate + NAD+ | - |
Cyberlindnera jadinii | D-ribulose 5-phosphate + CO2 + NADH | - |
? | |
6-phospho-D-gluconate + NADP+ | - |
Cyberlindnera jadinii | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Homo sapiens | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Rattus norvegicus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Geobacillus stearothermophilus | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Neurospora crassa | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r | |
6-phospho-D-gluconate + NADP+ | - |
Geobacillus stearothermophilus NCA 1503 | D-ribulose 5-phosphate + CO2 + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Cyberlindnera jadinii |
dimer | - |
Homo sapiens |
dimer | - |
Rattus norvegicus |
dimer | - |
Neurospora crassa |
dimer | 2 * 51000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable at 60°C | Geobacillus stearothermophilus |
70 | - |
denaturation half-time of 80 min | Geobacillus stearothermophilus |
80 | - |
denaturation half-time of 3 min | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Cyberlindnera jadinii |
8 | - |
- |
Homo sapiens |
8 | - |
- |
Rattus norvegicus |
8 | - |
- |
Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Cyberlindnera jadinii | |
NAD+ | - |
Rattus norvegicus | |
NAD+ | some activity with NAD+ | Geobacillus stearothermophilus | |
NADP+ | - |
Cyberlindnera jadinii | |
NADP+ | - |
Rattus norvegicus | |
NADP+ | - |
Geobacillus stearothermophilus | |
NADP+ | - |
Neurospora crassa |