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Literature summary for 1.1.1.431 extracted from

  • Khattab, S.M.; Saimura, M.; Kodaki, T.
    Boost in bioethanol production using recombinant Saccharomyces cerevisiae with mutated strictly NADPH-dependent xylose reductase and NADP+-dependent xylitol dehydrogenase (2013), J. Biotechnol., 165, 153-156.
    View publication on PubMed
Search for more literature for 1.1.1.431

Cloned(Commentary)

Cloned (Comment) Organism
recombinant overexpression of strictly NADPH-dependent xylose reductase with mutated strictly NADP+-dependent xylitol dehydrogenase leading to increased bioethanol production and decreased xylitol accumulation in Saccharomyces cerevisiae strain D452-2 resulting in mutant strains SK-N1 to SK-N4 Scheffersomyces stipitis

Protein Variants

Protein Variants Comment Organism
additional information construction of a set of recombinant Saccharomyces cerevisiae carrying a mutated strictly NADPH-dependent xylose reductase and NADP+-dependent xylitol dehydrogenase genes with overexpression of endogenous xylulokinase (XK), effects of complete NADPH/NADP+ recycling on ethanol fermentation and xylitol accumulation, overview. The mutated strains demonstrate 0% and 10% improvement in ethanol production, and reduced xylitol accumulation, ranging 34.4-54.7% compared with the control strain Scheffersomyces stipitis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Scheffersomyces stipitis
-
 D-xylose + NADH + H+
-
 r
xylitol + NADP+ Scheffersomyces stipitis
-
 D-xylose + NADPH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Scheffersomyces stipitis P31867 i.e Pichia stipitis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11 2.52 recombinant strains harboring genes xyl1 and mutated XDH, pH and temperature not specified in the publication, substrates D-xylosse and NADPH Scheffersomyces stipitis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
-
 Scheffersomyces stipitis D-xylose + NADH + H+
-
 r
xylitol + NADP+
-
 Scheffersomyces stipitis D-xylose + NADPH + H+
-
 r

Synonyms

Synonyms Comment Organism
NAD(P)H-dependent D-xylose reductase
-
 Scheffersomyces stipitis
NADPH-dependent xylose reductase
-
 Scheffersomyces stipitis
XYL1
-
 Scheffersomyces stipitis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Scheffersomyces stipitis
NADH
-
 Scheffersomyces stipitis
NADP+
-
 Scheffersomyces stipitis
NADPH
-
 Scheffersomyces stipitis

General Information

General Information Comment Organism
metabolism the redox balance between xylose reductase (XR) and xylitol dehydrogenase (XDH, EC 1.1.1.10) is thought to be an important factor in effective xylose fermentation Scheffersomyces stipitis
physiological function strictly NADPH-dependent xylose reductase with mutated strict NADP+-dependent xylitol dehydrogenase, EC 1.1.1.10, are more effective in increasing bioethanol production and decreasing xylitol accumulation than the wild-type, overview Scheffersomyces stipitis