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Literature summary for 1.1.1.431 extracted from
- Production of xylitol from D-xylose and glucose with recombinant Corynebacterium glutamicum (2010), Enzyme Microb. Technol., 46, 366-371.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene XYL1 | Corynebacterium glutamicum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | production of xylitol from D-xylose and D-glucose with recombinant Corynebacterium glutamicum, the strain is engineered to express the xylose reductase gene XYL1of Pichia stipitis, and produces xylose reductase with a specific activity of ca. 0.6 U/mg protein. Due to the absence of xylose isomerase and xylitol dehydrogenase genes, loose catabolite repression, high NADPH regeneration capacity, and tolerance against sugar-induced osmotic stress, the recombinant biocatalyst is able to efficiently produce xylitol from D-xylose using glucose as source of reducing equivalents | Corynebacterium glutamicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylose + NADPH + H+ | Corynebacterium glutamicum | - |
xylitol + NADP+ | - |
? |  |
| D-xylose + NADPH + H+ | Corynebacterium glutamicum ATCC 13032 | - |
xylitol + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
gene XYL1 | - |
| Corynebacterium glutamicum ATCC 13032 | - |
gene XYL1 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylose + NADPH + H+ | - |
Corynebacterium glutamicum | xylitol + NADP+ | - |
? | |
| D-xylose + NADPH + H+ | - |
Corynebacterium glutamicum ATCC 13032 | xylitol + NADP+ | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Corynebacterium glutamicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Corynebacterium glutamicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Corynebacterium glutamicum | |
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