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Literature summary for 1.1.1.430 extracted from
- Improving ethanol and xylitol fermentation at elevated temperature through substitution of xylose reductase in Kluyveromyces marxianus (2013), J. Ind. Microbiol. Biotechnol., 40, 305-316.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| construction of Kluyveromyces marxianus strains expressing Pichia stipitis Psxyl1 genes leading to reversed cofactor specificity and ethanol and xylitol production | Kluyveromyces marxianus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | the native xylose reductase gene Kmxyl1 of the Kluyveromyces marxianus strain YHJ010 is substituted with xylose reductase or its mutants N272D, K21A/N272D, or K270M from Pichia stipitis, i.e. Scheffersomyces stipitis, resulting in Kluyveromyces marxianus strains YZB013, YZB014, and YZB015. The ability of the resultant recombinant strains to assimilate xylose to produce xylitol and ethanol at elevated temperature is greatly improved, overview. But the strain YZB015 expressing a mutant PsXR K21A/N272D, with which co-enzyme preference is completely reversed from NADPH to NADH, fails to ferment due to the low expression | Kluyveromyces marxianus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Kluyveromyces marxianus | - |
- |
- |
| Kluyveromyces marxianus YHJ010 | - |
- |
- |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADPH-preferring xylose reductase | - |
Kluyveromyces marxianus |
| xylose reductase | - |
Kluyveromyces marxianus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Kluyveromyces marxianus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Kluyveromyces marxianus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Kluyveromyces marxianus strains expressing Pichia stipitis Psxyl1 genes show reversed cofactor specificity, overview | Kluyveromyces marxianus | |
| NAD+ | - |
Kluyveromyces marxianus |  |
| NADH | - |
Kluyveromyces marxianus | |
| NADP+ | - |
Kluyveromyces marxianus | |
| NADPH | preferred cofactor | Kluyveromyces marxianus | |
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Release 2023.1 (January 2023)
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