Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 1 mM, increases activity by 13% | Candida parapsilosis | |
cysteine | 1 mM, increases activity by 29% | Candida parapsilosis | |
dithiothreitol | 1 mM, increases activity by 43% | Candida parapsilosis | |
glutathione | 1 mM, increases activity by 21% | Candida parapsilosis | |
additional information | neither inhibited nor activated by EDTA at concentrations ranging from 1 to 10 mM | Candida parapsilosis |
Cloned (Comment) | Organism |
---|---|
expression in Candida tropicalis increases production of ethanol and glycerol | Candida parapsilosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | activity is completely restored by addition of EDTA | Candida parapsilosis | |
NAD+ | competitive with NADH, non-competitive with D-xylose | Candida parapsilosis | |
xylitol | non-competitive against NADH and D-xylose | Candida parapsilosis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
NADH | pH 6.0 | Candida parapsilosis | |
0.0365 | - |
NADPH | pH 6.0 | Candida parapsilosis | |
31.5 | - |
D-xylose | pH 6.0, coenzyme: NADH | Candida parapsilosis | |
151.7 | - |
D-erythrose | pH 6.0 | Candida parapsilosis | |
244.3 | - |
D-xylose | pH 6.0, coenzyme: NADPH | Candida parapsilosis | |
285.4 | - |
D-arabinose | pH 6.0 | Candida parapsilosis | |
302 | - |
D-ribose | pH 6.0 | Candida parapsilosis | |
326.3 | - |
xylitol | pH 6.0 | Candida parapsilosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 mM, stimulates | Candida parapsilosis | |
CoCl2 | 1 mM, stimulates | Candida parapsilosis | |
FeCl2 | 1 mM, stimulates | Candida parapsilosis | |
MgCl2 | 1 mM, stimulates | Candida parapsilosis | |
MnCl2 | 1 mM, stimulates | Candida parapsilosis | |
additional information | neither inhibited nor activated by EDTA at concentrations ranging from 1 to 10 mM | Candida parapsilosis | |
NiCl2 | 1 mM, stimulates | Candida parapsilosis | |
ZnCl2 | 1 mM, stimulates | Candida parapsilosis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36400 | - |
2 * 36400, SDS-PAGE | Candida parapsilosis |
36629 | - |
2 * 36629, calculated from sequence | Candida parapsilosis |
69000 | - |
gel filtration | Candida parapsilosis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Candida parapsilosis | Q6Y0Z3 | KFCC-10875 | - |
Candida parapsilosis KFCC-10875 | Q6Y0Z3 | KFCC-10875 | - |
Purification (Comment) | Organism |
---|---|
- |
Candida parapsilosis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
41.7 | - |
- |
Candida parapsilosis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabinose + NADPH + H+ | - |
Candida parapsilosis | D-arabinitol + NADP+ | - |
? | |
D-arabinose + NADPH + H+ | - |
Candida parapsilosis KFCC-10875 | D-arabinitol + NADP+ | - |
? | |
D-erythrose + NADPH + H+ | - |
Candida parapsilosis | D-erythritol + NADP+ | - |
? | |
D-erythrose + NADPH + H+ | - |
Candida parapsilosis KFCC-10875 | D-erythritol + NADP+ | - |
? | |
D-ribose + NADPH + H+ | - |
Candida parapsilosis | ? | - |
? | |
D-ribose + NADPH + H+ | - |
Candida parapsilosis KFCC-10875 | ? | - |
? | |
D-xylose + NADH + H+ | the enzyme specifically transfers the 4-pro-R hydrogen from the C-4 of the nicotinamide ring to the re face of the carbonyl carbon of the substrate | Candida parapsilosis | xylitol + NAD+ | - |
r | |
D-xylose + NADH + H+ | the enzyme specifically transfers the 4-pro-R hydrogen from the C-4 of the nicotinamide ring to the re face of the carbonyl carbon of the substrate | Candida parapsilosis KFCC-10875 | xylitol + NAD+ | - |
r | |
D-xylose + NADPH + H+ | - |
Candida parapsilosis | xylitol + NADP+ | - |
? | |
D-xylose + NADPH + H+ | - |
Candida parapsilosis KFCC-10875 | xylitol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36400, SDS-PAGE | Candida parapsilosis |
dimer | 2 * 36629, calculated from sequence | Candida parapsilosis |
Synonyms | Comment | Organism |
---|---|---|
XYL1 | - |
Candida parapsilosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Candida parapsilosis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
60 days, 50% loss of activity | Candida parapsilosis |
20 | - |
8 days, 50% loss of activity | Candida parapsilosis |
30 | - |
3 days, 50% loss of activity | Candida parapsilosis |
45 | - |
4.5 h, 50% loss of activity | Candida parapsilosis |
50 | - |
2 min, 50% loss of activity | Candida parapsilosis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of D-xylose | Candida parapsilosis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | pH 5.0: 81% of maximal activity, pH 7.0: about 65% of maximal activity | Candida parapsilosis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | strongly prefers NADH to NADPH | Candida parapsilosis | |
NADPH | strongly prefers NADH to NADPH | Candida parapsilosis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
Cu2+ | pH 6.0 | Candida parapsilosis | |
0.18 | - |
NAD+ | pH 6.0, competitive with NADH | Candida parapsilosis | |
0.325 | - |
NAD+ | pH 6.0, non-competitive with D-xylose | Candida parapsilosis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Candida parapsilosis | calculated from sequence | - |
5.19 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.019 | - |
D-xylose | pH 6.0, coenzyme: NADPH | Candida parapsilosis | |
0.028 | - |
D-arabinose | pH 6.0 | Candida parapsilosis | |
0.031 | - |
xylitol | pH 6.0 | Candida parapsilosis | |
0.043 | - |
D-ribose | pH 6.0 | Candida parapsilosis | |
0.089 | - |
D-erythrose | pH 6.0 | Candida parapsilosis | |
1.5 | - |
D-xylose | pH 6.0, coenzyme: NADH | Candida parapsilosis | |
127 | - |
NADPH | pH 6.0 | Candida parapsilosis | |
13900 | - |
NADH | pH 6.0 | Candida parapsilosis |