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Literature summary for 1.1.1.43 extracted from

  • Lee, Y.N.; Lessie, T.G.
    Purification and characterization of the two 6-phosphogluconate dehydrogenase species from Pseudomonas multivorans (1974), J. Bacteriol., 120, 1043-1057.
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
adenosine 5'-triphosphate ATP inhibits the enzyme which is active with NAD+, not the NADP+ dependent activity Burkholderia cepacia
ATP NAD+-specific enzyme Enterococcus faecalis
fructose 1,6-bisphosphate NADP+-specific enzyme Enterococcus faecalis
guanosine 5'-triphosphate GTP inhibits the NADP+ specific enzyme Burkholderia cepacia
NADPH inhibition is competitive with respect to NADP+ Burkholderia cepacia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
6-phospho-D-gluconate
-
Burkholderia cepacia
0.02
-
NADP+ enzyme active with NAD+ and NADP+-specific enzyme Burkholderia cepacia
0.2
-
NAD+
-
Burkholderia cepacia
0.2
-
6-phospho-D-gluconate
-
Burkholderia cepacia
0.3
-
6-phospho-D-gluconate enzyme active with NAD+ or NADP+ Burkholderia cepacia
0.7
-
6-phospho-D-gluconate
-
Burkholderia cepacia
1
-
6-phospho-D-gluconate 0.005 M GTP Burkholderia cepacia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
2 * 40000, enzyme active with NAD+ Burkholderia cepacia
57000
-
2 * 57000 Neurospora crassa
60000
-
2 * 60000, enzyme specific for NADP+ Burkholderia cepacia
82000
-
enzyme active with NAD+, sucrose density gradient sedimentation Burkholderia cepacia
84000
-
enzyme active with NAD+, gel filtration Burkholderia cepacia
110000 120000 NADP+-specific enzyme Neurospora crassa
120000
-
NADP+-specific enzyme, gel filtration Burkholderia cepacia
123000
-
NADP+-specific enzyme, sucrose density gradient sedimentation Burkholderia cepacia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6-phospho-D-gluconate + NAD(P)+ Neurospora crassa
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
r
6-phospho-D-gluconate + NAD(P)+ Pseudomonas aeruginosa
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
r
6-phospho-D-gluconate + NAD(P)+ Enterococcus faecalis
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
r
6-phospho-D-gluconate + NAD(P)+ Burkholderia cepacia
-
6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
r

Organism

Organism UniProt Comment Textmining
Burkholderia cepacia
-
ATCC 17616, synonymous with Pseudomonas cepacia
-
Enterococcus faecalis
-
-
-
Neurospora crassa
-
-
-
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Burkholderia cepacia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
34
-
NADP+ as cofactor Burkholderia cepacia
42.3
-
NAD+ as cofactor Burkholderia cepacia

Storage Stability

Storage Stability Organism
0°C both 6-phosphogluconate dehydrogenase species obtained by treatment with ammonium sulfate retain 100% of their activity when stored for several months Burkholderia cepacia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6-phospho-D-gluconate + NAD(P)+
-
Neurospora crassa 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
r
6-phospho-D-gluconate + NAD(P)+
-
Pseudomonas aeruginosa 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
r
6-phospho-D-gluconate + NAD(P)+
-
Enterococcus faecalis 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
r
6-phospho-D-gluconate + NAD(P)+
-
Burkholderia cepacia 6-phospho-2-dehydro-D-gluconate + NAD(P)H
-
r
D-glucose-6-phosphate + NAD+ enzyme active with NAD+, 2% of the activity compared to 6-phosphogluconate when NAD+ is cofactor, no activity detected with NADP+ as cofactor Burkholderia cepacia 2-dehydro-6-phospho-D-glucose + NADH
-
?
D-glucose-6-phosphate + NADP+ NADP-specific enzyme, 4% of the activity with 6-phosphogluconate Burkholderia cepacia 2-dehydro-6-phospho-D-glucose + NADPH
-
?
additional information appears to lack 6PGAD except during growth on glucose or gluconate Pseudomonas aeruginosa ?
-
?
additional information glucose, 2-deoxyglucose, gluconate, fructose-6-phosphate, glucose-1-phosphate, fructose-1,6-diphosphate, ribulose-5-phosphate and 1,3-diphosphoglycerate are no substrates Burkholderia cepacia ?
-
?

Subunits

Subunits Comment Organism
dimer 2 * 40000, enzyme active with NAD+ Burkholderia cepacia
dimer 2 * 60000, enzyme specific for NADP+ Burkholderia cepacia
dimer 2 * 57000 Neurospora crassa

Synonyms

Synonyms Comment Organism
6PGAD
-
Pseudomonas aeruginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
NADP-specific enzyme Burkholderia cepacia
8.5 9.5 enzyme active with NAD+ Burkholderia cepacia

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Burkholderia cepacia