Literature summary for 1.1.1.429 extracted from

von Horsten, S.; Lippert, M.L.; Geisselbrecht, Y.; Schuehle, K.; Schall, I.; Essen, L.O.; Heider, J.
Inactive pseudoenzyme subunits in heterotetrameric BbsCD, a novel short-chain alcohol dehydrogenase involved in anaerobic toluene degradation (2022), FEBS J., 289, 1023-1042 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Thauera aromatica

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 0.2 M Mg (CH3COO)2, 0.1 M sodium cacodylate, pH 6.5, and 20% (w/v) PEG 8000	Thauera aromatica

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.096	-	(S)-2-benzoylsuccinyl-CoA	at pH 6.2 and 30°C	Thauera aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-2-benzoylsuccinyl-CoA + NADH + H+	Thauera aromatica	-	(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA + NAD+	-	?

Organism

Organism	UniProt	Comment	Textmining
Thauera aromatica	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE column chromatography and hydroxyapatite column chromatography	Thauera aromatica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.28	-	crude extract, at pH 6.2 and 30°C	Thauera aromatica
0.86	-	after 3.1 enrichment, at pH 6.2 and 30°C	Thauera aromatica

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-2-benzoylsuccinyl-CoA + NADH + H+	-	Thauera aromatica	(2S)-[(R)-hydroxy(phenyl)methyl]succinyl-CoA + NAD+	-	?
additional information	the enzyme does not use NADP+ as electron acceptor	Thauera aromatica	?	-	-

Subunits

Subunits	Comment	Organism
heterotetramer	2 * 26000 + 2 * 28000, SDS-PAGE	Thauera aromatica

Synonyms

Synonyms	Comment	Organism
(S,R)-2-(alpha-hydroxybenzyl)succinyl-CoA dehydrogenase	-	Thauera aromatica
bbsCD	-	Thauera aromatica

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Release 2023.1 (January 2023)