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Literature summary for 1.1.1.427 extracted from
- D-arabinose dehydrogenase and biosynthesis of erythroascorbic acid in Candida albicans (1996), Biochim. Biophys. Acta, 1297, 1-8 .
General Stability
| General Stability | Organism |
|---|---|
| prolonged storage at 4°C, freezing and thawing, and lyophilisation of enzyme solutions result in significant losses of the enzyme activity | Candida albicans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoic acid | 75% residual activity at 1 mM | Candida albicans |  |
| Cu2+ | 69% residual activity at 1 mM | Candida albicans | |
| Fe2+ | 37% residual activity at 1 mM | Candida albicans | |
| Hg2+ | complete inhibition at 1 mM | Candida albicans | |
| Mg2+ | 85% residual activity at 1 mM | Candida albicans | |
| Mn2+ | 87% residual activity at 1 mM | Candida albicans | |
| additional information | not inhibited by EDTA and dithiothreitol | Candida albicans | |
| N-ethylmaleimide | 54% residual activity at 1 mM | Candida albicans | |
| NADPH | NADPH inhibits the enzyme activity competitively with respect to NADP+ | Candida albicans | |
| Zn2+ | 48% residual activity at 1 mM | Candida albicans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0446 | - |
NADP+ | with D-arabinose as cosubstrate, at pH 8.0 and 30°C | Candida albicans | |
| 28.9 | - |
L-fucose | at pH 8.0 and 30°C | Candida albicans | |
| 29.2 | - |
D-arabinose | at pH 8.0 and 30°C | Candida albicans | |
| 37.1 | - |
L-Xylose | at pH 8.0 and 30°C | Candida albicans | |
| 91.3 | - |
L-galactose | at pH 8.0 and 30°C | Candida albicans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Candida albicans | 5829 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not influenced by Ca2+ | Candida albicans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
gel filtration | Candida albicans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
| Candida albicans ATCC 10231 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sepharose A-50, Sephacryl S-200, Cibacron blue and phenyl-Sepharose CL-4B chromatographies | Candida albicans |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 20% (v/v) glycerol, at least 2 months, no loss of activity | Candida albicans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-arabinose + NADP+ | - |
Candida albicans | D-arabinono-1,4-1actone + NADPH + H+ | - |
? | |
| D-arabinose + NADP+ | - |
Candida albicans ATCC 10231 | D-arabinono-1,4-1actone + NADPH + H+ | - |
? | |
| L-fucose + NADP+ | - |
Candida albicans | ? | - |
? | |
| L-fucose + NADP+ | - |
Candida albicans ATCC 10231 | ? | - |
? | |
| L-galactose + NADP+ | - |
Candida albicans | ? | - |
? | |
| L-galactose + NADP+ | - |
Candida albicans ATCC 10231 | ? | - |
? | |
| L-xylose + NADP+ | - |
Candida albicans | ? | - |
? | |
| L-xylose + NADP+ | - |
Candida albicans ATCC 10231 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Candida albicans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| D-arabinose dehydrogenase | - |
Candida albicans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Candida albicans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 50 | the enzyme loses 50% of its activity at 35°C for 60 min and is completely inactivated at 50°C in 30 min | Candida albicans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 53.2 | - |
L-galactose | at pH 8.0 and 30°C | Candida albicans | |
| 63.4 | - |
L-Xylose | at pH 8.0 and 30°C | Candida albicans | |
| 68.2 | - |
D-arabinose | at pH 8.0 and 30°C | Candida albicans | |
| 69.5 | - |
L-fucose | at pH 8.0 and 30°C | Candida albicans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Candida albicans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7.5 | the enzyme is relatively stable from pH 4.5 to 7.5 after 24 h incubation | Candida albicans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Candida albicans | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Candida albicans | isoelectric focusing | - |
5 |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.0786 | - |
at pH 8.0 and 30°C | Candida albicans | NADPH | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.58 | - |
L-galactose | at pH 8.0 and 30°C | Candida albicans | |
| 1.71 | - |
L-Xylose | at pH 8.0 and 30°C | Candida albicans | |
| 2.34 | - |
D-arabinose | at pH 8.0 and 30°C | Candida albicans | |
| 2.4 | - |
L-fucose | at pH 8.0 and 30°C | Candida albicans | |
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