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Literature summary for 1.1.1.427 extracted from

  • Brouns, S.J.; Turnbull, A.P.; Willemen, H.L.; Akerboom, J.; van der Oost, J.
    Crystal structure and biochemical properties of the D-arabinose dehydrogenase from Sulfolobus solfataricus (2007), J. Mol. Biol., 371, 1249-1260.
    View publication on PubMed
Search for more literature for 1.1.1.427

Crystallization (Commentary)

Crystallization (Comment) Organism
the structure is solved to a resolution of 1.80 A by single-wavelength anomalous diffraction and phased using the two endogenous zinc ions per subunit. The structure reveals a catalytic and cofactor binding domain Saccharolobus solfataricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.038
-
 NADP+ pH 7.5, 70°C, determined with 10 mM D-arabinose Saccharolobus solfataricus
0.22
-
 L-fucose pH 7.5, 70°C, determined with 0.4 mM NADP+ Saccharolobus solfataricus
1.25
-
 NAD+ pH 7.5, 70°C, determined with 10 mM D-arabinose Saccharolobus solfataricus
1.4
-
 D-arabinose pH 7.5, 70°C, determined with 0.4 mM NADP+ Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains two endogenous zinc ions per subunit.A zinc ion is bound in the loop that protrudes from the catalytic domain. This zinc is tetrahedrally coordinated by residues D94, C97, C100 and C108 Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus Q97YM2
-
-
Saccharolobus solfataricus P2 Q97YM2
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinose + NAD+
-
 Saccharolobus solfataricus D-arabinono-1,4-lactone + NADH + H+
-
 ?
D-arabinose + NAD+
-
 Saccharolobus solfataricus P2 D-arabinono-1,4-lactone + NADH + H+
-
 ?
D-arabinose + NADP+
-
 Saccharolobus solfataricus D-arabinono-1,4-lactone + NADPH + H+
-
 ?
D-arabinose + NADP+
-
 Saccharolobus solfataricus P2 D-arabinono-1,4-lactone + NADPH + H+
-
 ?
D-ribose + NADP+
-
 Saccharolobus solfataricus D-ribonolactone + NADPH
-
 ?
L-fucose + NADP+
-
 Saccharolobus solfataricus L-fuconolactone + NADPH + H+
-
 ?
L-fucose + NADP+
-
 Saccharolobus solfataricus P2 L-fuconolactone + NADPH + H+
-
 ?
L-galactose + NADP+
-
 Saccharolobus solfataricus L-galactonolactone + NADPH
-
 ?
additional information the enzyme is specific for sugars with D-arabinose-type hydroxyl group stereo-configurations at C3 and C4. Negligible activity with D-glucose, D-galactose, D-fucose, L-arabinose and D-xylose Saccharolobus solfataricus ?
-
 ?
additional information the enzyme is specific for sugars with D-arabinose-type hydroxyl group stereo-configurations at C3 and C4. Negligible activity with D-glucose, D-galactose, D-fucose, L-arabinose and D-xylose Saccharolobus solfataricus P2 ?
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
AraDH
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
 assay at Saccharolobus solfataricus
91
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 100 70°C: about 40% of maximal activity, 100°C: about 90% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
85
-
 half-life: 42 min Saccharolobus solfataricus
90
-
 half-life: 26 min Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Saccharolobus solfataricus
8.2
-
-
 Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
7.1 9.1 pH 7.1: about 40% of maximal activity, pH 9.1: about 60% of maximal activity, pH 9.5: about 30% of maximal activity Saccharolobus solfataricus

Cofactor

Cofactor Comment Organism Structure
NAD+ the catalytic efficiency of NADP+ in combination with D-arabinose is 36-fold higher than that of NAD+ Saccharolobus solfataricus
NADP+ the catalytic efficiency of NADP+ in combination with D-arabinose is 36-fold higher than that of NAD+ Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
10.6
-
 D-arabinose pH 7.5, 70°C, determined with 0.4 mM NADP+ Saccharolobus solfataricus
14.5
-
 NAD+ pH 7.5, 70°C, determined with 10 mM D-arabinose Saccharolobus solfataricus
67
-
 L-fucose pH 7.5, 70°C, determined with 0.4 mM NADP+ Saccharolobus solfataricus
525
-
 NADP+ pH 7.5, 70°C, determined with 10 mM D-arabinose Saccharolobus solfataricus