BRENDA - Enzyme Database
show all sequences of 1.1.1.424

D-Xylose degradation pathway in the halophilic archaeon Haloferax volcanii

Johnsen, U.; Dambeck, M.; Zaiss, H.; Fuhrer, T.; Soppa, J.; Sauer, U.; Schonheit, P.; J. Biol. Chem. 284, 27290-27303 (2009)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
NADP+
pH 7.5, 42°C
Haloferax volcanii
0.36
-
NAD+
pH 7.5, 42°C
Haloferax volcanii
4.7
-
D-xylose
pH 7.5, 42°C
Haloferax volcanii
198
-
D-glucose
pH 7.5, 42°C
Haloferax volcanii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
165000
-
gel filtration
Haloferax volcanii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-xylose + NAD+
Haloferax volcanii
-
D-xylonolactone + NADH + H+
-
-
?
D-xylose + NAD+
Haloferax volcanii DSM 3757
-
D-xylonolactone + NADH + H+
-
-
?
D-xylose + NADP+
Haloferax volcanii
-
D-xylonolactone + NADPH + H+
-
-
?
D-xylose + NADP+
Haloferax volcanii DSM 3757
-
D-xylonolactone + NADPH + H+
-
-
?
additional information
Haloferax volcanii
no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
?
-
-
-
additional information
Haloferax volcanii DSM 3757
no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Haloferax volcanii
D4GP29
-
-
Haloferax volcanii DSM 3757
D4GP29
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.8
-
substrate D-glucose, pH 7.5, 42°C
Haloferax volcanii
24.1
-
substrate D-xylose, pH 7.5, 42°C
Haloferax volcanii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-glucose + NADP+
-
757140
Haloferax volcanii
D-glucoselactone + NADPH + H+
-
-
-
?
D-glucose + NADP+
-
757140
Haloferax volcanii DSM 3757
D-glucoselactone + NADPH + H+
-
-
-
?
D-xylose + NAD+
-
757140
Haloferax volcanii
D-xylonolactone + NADH + H+
-
-
-
?
D-xylose + NAD+
-
757140
Haloferax volcanii DSM 3757
D-xylonolactone + NADH + H+
-
-
-
?
D-xylose + NADP+
-
757140
Haloferax volcanii
D-xylonolactone + NADPH + H+
-
-
-
?
D-xylose + NADP+
-
757140
Haloferax volcanii DSM 3757
D-xylonolactone + NADPH + H+
-
-
-
?
additional information
no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
757140
Haloferax volcanii
?
-
-
-
-
additional information
no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
757140
Haloferax volcanii DSM 3757
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 55000, SDS-PAGE
Haloferax volcanii
Synonyms
Synonyms
Commentary
Organism
HVO_B0028
-
Haloferax volcanii
xacA
-
Haloferax volcanii
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
NADP+ is preferred over NAD+
Haloferax volcanii
NADP+
NADP+ is preferred over NAD+
Haloferax volcanii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
NADP+ is preferred over NAD+
Haloferax volcanii
NADP+
NADP+ is preferred over NAD+
Haloferax volcanii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
NADP+
pH 7.5, 42°C
Haloferax volcanii
0.36
-
NAD+
pH 7.5, 42°C
Haloferax volcanii
4.7
-
D-xylose
pH 7.5, 42°C
Haloferax volcanii
198
-
D-glucose
pH 7.5, 42°C
Haloferax volcanii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
165000
-
gel filtration
Haloferax volcanii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-xylose + NAD+
Haloferax volcanii
-
D-xylonolactone + NADH + H+
-
-
?
D-xylose + NAD+
Haloferax volcanii DSM 3757
-
D-xylonolactone + NADH + H+
-
-
?
D-xylose + NADP+
Haloferax volcanii
-
D-xylonolactone + NADPH + H+
-
-
?
D-xylose + NADP+
Haloferax volcanii DSM 3757
-
D-xylonolactone + NADPH + H+
-
-
?
additional information
Haloferax volcanii
no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
?
-
-
-
additional information
Haloferax volcanii DSM 3757
no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
?
-
-
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
7.8
-
substrate D-glucose, pH 7.5, 42°C
Haloferax volcanii
24.1
-
substrate D-xylose, pH 7.5, 42°C
Haloferax volcanii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-glucose + NADP+
-
757140
Haloferax volcanii
D-glucoselactone + NADPH + H+
-
-
-
?
D-glucose + NADP+
-
757140
Haloferax volcanii DSM 3757
D-glucoselactone + NADPH + H+
-
-
-
?
D-xylose + NAD+
-
757140
Haloferax volcanii
D-xylonolactone + NADH + H+
-
-
-
?
D-xylose + NAD+
-
757140
Haloferax volcanii DSM 3757
D-xylonolactone + NADH + H+
-
-
-
?
D-xylose + NADP+
-
757140
Haloferax volcanii
D-xylonolactone + NADPH + H+
-
-
-
?
D-xylose + NADP+
-
757140
Haloferax volcanii DSM 3757
D-xylonolactone + NADPH + H+
-
-
-
?
additional information
no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
757140
Haloferax volcanii
?
-
-
-
-
additional information
no substrates: L-arabinose, D-arabinose, D-ribose, D-mannose, and L-mannose
757140
Haloferax volcanii DSM 3757
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 55000, SDS-PAGE
Haloferax volcanii
General Information
General Information
Commentary
Organism
physiological function
D-xylose is oxidized exclusively to the tricarboxylic acid cycle intermediate alpha-ketoglutarate, involving D-xylose dehydrogenase (HVO_B0028), a xylonate dehydratase (HVO_B0038A), 2-keto-3-deoxyxylonate dehydratase (HVO_B0027), and alpha-ketoglutarate semialdehyde dehydrogenase (HVO_B0039). An HVO_B0028 gene deletion mutant cannot grow on xylose but grows unaffected on glucose as the wild-type
Haloferax volcanii
General Information (protein specific)
General Information
Commentary
Organism
physiological function
D-xylose is oxidized exclusively to the tricarboxylic acid cycle intermediate alpha-ketoglutarate, involving D-xylose dehydrogenase (HVO_B0028), a xylonate dehydratase (HVO_B0038A), 2-keto-3-deoxyxylonate dehydratase (HVO_B0027), and alpha-ketoglutarate semialdehyde dehydrogenase (HVO_B0039). An HVO_B0028 gene deletion mutant cannot grow on xylose but grows unaffected on glucose as the wild-type
Haloferax volcanii
Other publictions for EC 1.1.1.424
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750559
Sutter
Characterization of a pentono ...
Haloferax volcanii, Haloferax volcanii DSM 3757
FEMS Microbiol. Lett.
364
fnx140
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
757140
Johnsen
D-Xylose degradation pathway ...
Haloferax volcanii, Haloferax volcanii DSM 3757
J. Biol. Chem.
284
27290-27303
2009
-
-
-
-
-
-
-
4
-
-
1
6
-
3
-
-
-
-
-
-
2
-
8
1
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
1
6
-
-
-
-
-
-
2
-
8
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
757089
Johnsen
Novel xylose dehydrogenase in ...
Haloarcula marismortui, Haloarcula marismortui DSM 3752
J. Bacteriol.
186
6198-6207
2004
3
-
1
-
-
-
-
5
-
-
1
4
-
2
-
-
1
-
-
-
1
-
8
1
2
1
-
-
3
1
2
-
2
-
-
-
3
-
1
2
-
-
-
-
-
-
5
-
-
1
4
-
-
-
1
-
-
1
-
8
1
1
-
-
3
1
2
-
-
-
-
-
-
3
3