BRENDA - Enzyme Database
show all sequences of 1.1.1.424

Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortui

Johnsen, U.; Schonheit, P.; J. Bacteriol. 186, 6198-6207 (2004)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
KCl
maximum activity at 1.5 M, strong stimulation
Haloarcula marismortui
MgCl2
maximum activity at 0.1 M, strong stimulation
Haloarcula marismortui
NaCl
maximum activity at 1.5 M, strong stimulation
Haloarcula marismortui
Cloned(Commentary)
Cloned (Commentary)
Organism
expression in Escherichia coli
Haloarcula marismortui
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
NADP+
pH 8.3, 37°C
Haloarcula marismortui
0.9
-
NAD+
pH 8.3, 37°C
Haloarcula marismortui
1.2
-
D-xylose
pH 8.3, 37°C
Haloarcula marismortui
2.3
-
D-ribose
pH 8.3, 37°C
Haloarcula marismortui
64
-
D-glucose
pH 8.3, 37°C
Haloarcula marismortui
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
175000
-
gel filtration
Haloarcula marismortui
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-xylose + NAD+
Haloarcula marismortui
-
D-xylonolactone + H+ + NADH
-
-
?
D-xylose + NAD+
Haloarcula marismortui DSM 3752
-
D-xylonolactone + H+ + NADH
-
-
?
D-xylose + NADP+
Haloarcula marismortui
-
D-xylonolactone + H+ + NADPH
-
-
?
D-xylose + NADP+
Haloarcula marismortui DSM 3752
-
D-xylonolactone + H+ + NADPH
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Haloarcula marismortui
Q5UY95
-
-
Haloarcula marismortui DSM 3752
Q5UY95
-
-
Purification (Commentary)
Purification (Commentary)
Organism
both native and recombinant protein
Haloarcula marismortui
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
100
-
pH 8.3, 37°C
Haloarcula marismortui
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-glucose + NADP+
-
757089
Haloarcula marismortui
D-gluconolactone + H+ + NADPH
-
-
-
?
D-glucose + NADP+
-
757089
Haloarcula marismortui DSM 3752
D-gluconolactone + H+ + NADPH
-
-
-
?
D-ribose + NADP+
-
757089
Haloarcula marismortui
D-ribonolactone + H+ + NADPH
-
-
-
?
D-ribose + NADP+
-
757089
Haloarcula marismortui DSM 3752
D-ribonolactone + H+ + NADPH
-
-
-
?
D-xylose + NAD+
-
757089
Haloarcula marismortui
D-xylonolactone + H+ + NADH
-
-
-
?
D-xylose + NAD+
-
757089
Haloarcula marismortui DSM 3752
D-xylonolactone + H+ + NADH
-
-
-
?
D-xylose + NADP+
-
757089
Haloarcula marismortui
D-xylonolactone + H+ + NADPH
-
-
-
?
D-xylose + NADP+
-
757089
Haloarcula marismortui DSM 3752
D-xylonolactone + H+ + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 57000, SDS-PAGE, 4 * 39900, calculated from sequence
Haloarcula marismortui
Synonyms
Synonyms
Commentary
Organism
Gfo6
-
Haloarcula marismortui
xacA
-
Haloarcula marismortui
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
presence of 1.5 M KCl
Haloarcula marismortui
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
273
-
D-glucose
pH 8.3, 37°C
Haloarcula marismortui
356
-
D-xylose
pH 8.3, 37°C
Haloarcula marismortui
360
-
D-ribose
pH 8.3, 37°C
Haloarcula marismortui
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
presence of 1.5 M KCl
Haloarcula marismortui
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
-
50% of maximum activity
Haloarcula marismortui
9
-
50% of maximum activity
Haloarcula marismortui
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Haloarcula marismortui
NADP+
-
Haloarcula marismortui
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
KCl
maximum activity at 1.5 M, strong stimulation
Haloarcula marismortui
MgCl2
maximum activity at 0.1 M, strong stimulation
Haloarcula marismortui
NaCl
maximum activity at 1.5 M, strong stimulation
Haloarcula marismortui
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Haloarcula marismortui
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Haloarcula marismortui
NADP+
-
Haloarcula marismortui
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
NADP+
pH 8.3, 37°C
Haloarcula marismortui
0.9
-
NAD+
pH 8.3, 37°C
Haloarcula marismortui
1.2
-
D-xylose
pH 8.3, 37°C
Haloarcula marismortui
2.3
-
D-ribose
pH 8.3, 37°C
Haloarcula marismortui
64
-
D-glucose
pH 8.3, 37°C
Haloarcula marismortui
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
175000
-
gel filtration
Haloarcula marismortui
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-xylose + NAD+
Haloarcula marismortui
-
D-xylonolactone + H+ + NADH
-
-
?
D-xylose + NAD+
Haloarcula marismortui DSM 3752
-
D-xylonolactone + H+ + NADH
-
-
?
D-xylose + NADP+
Haloarcula marismortui
-
D-xylonolactone + H+ + NADPH
-
-
?
D-xylose + NADP+
Haloarcula marismortui DSM 3752
-
D-xylonolactone + H+ + NADPH
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
both native and recombinant protein
Haloarcula marismortui
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
100
-
pH 8.3, 37°C
Haloarcula marismortui
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-glucose + NADP+
-
757089
Haloarcula marismortui
D-gluconolactone + H+ + NADPH
-
-
-
?
D-glucose + NADP+
-
757089
Haloarcula marismortui DSM 3752
D-gluconolactone + H+ + NADPH
-
-
-
?
D-ribose + NADP+
-
757089
Haloarcula marismortui
D-ribonolactone + H+ + NADPH
-
-
-
?
D-ribose + NADP+
-
757089
Haloarcula marismortui DSM 3752
D-ribonolactone + H+ + NADPH
-
-
-
?
D-xylose + NAD+
-
757089
Haloarcula marismortui
D-xylonolactone + H+ + NADH
-
-
-
?
D-xylose + NAD+
-
757089
Haloarcula marismortui DSM 3752
D-xylonolactone + H+ + NADH
-
-
-
?
D-xylose + NADP+
-
757089
Haloarcula marismortui
D-xylonolactone + H+ + NADPH
-
-
-
?
D-xylose + NADP+
-
757089
Haloarcula marismortui DSM 3752
D-xylonolactone + H+ + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 57000, SDS-PAGE, 4 * 39900, calculated from sequence
Haloarcula marismortui
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
50
-
presence of 1.5 M KCl
Haloarcula marismortui
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
273
-
D-glucose
pH 8.3, 37°C
Haloarcula marismortui
356
-
D-xylose
pH 8.3, 37°C
Haloarcula marismortui
360
-
D-ribose
pH 8.3, 37°C
Haloarcula marismortui
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.3
-
presence of 1.5 M KCl
Haloarcula marismortui
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
-
50% of maximum activity
Haloarcula marismortui
9
-
50% of maximum activity
Haloarcula marismortui
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.3
-
D-glucose
pH 8.3, 37°C
Haloarcula marismortui
157
-
D-ribose
pH 8.3, 37°C
Haloarcula marismortui
297
-
D-xylose
pH 8.3, 37°C
Haloarcula marismortui
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
4.3
-
D-glucose
pH 8.3, 37°C
Haloarcula marismortui
157
-
D-ribose
pH 8.3, 37°C
Haloarcula marismortui
297
-
D-xylose
pH 8.3, 37°C
Haloarcula marismortui
Other publictions for EC 1.1.1.424
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
750559
Sutter
Characterization of a pentono ...
Haloferax volcanii, Haloferax volcanii DSM 3757
FEMS Microbiol. Lett.
364
fnx140
2017
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
757140
Johnsen
D-Xylose degradation pathway ...
Haloferax volcanii, Haloferax volcanii DSM 3757
J. Biol. Chem.
284
27290-27303
2009
-
-
-
-
-
-
-
4
-
-
1
6
-
3
-
-
-
-
-
-
2
-
8
1
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
4
-
-
1
6
-
-
-
-
-
-
2
-
8
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
757089
Johnsen
Novel xylose dehydrogenase in ...
Haloarcula marismortui, Haloarcula marismortui DSM 3752
J. Bacteriol.
186
6198-6207
2004
3
-
1
-
-
-
-
5
-
-
1
4
-
2
-
-
1
-
-
-
1
-
8
1
2
1
-
-
3
1
2
-
2
-
-
-
3
-
1
2
-
-
-
-
-
-
5
-
-
1
4
-
-
-
1
-
-
1
-
8
1
1
-
-
3
1
2
-
-
-
-
-
-
3
3