Literature summary for 1.1.1.420 extracted from

Carter, M.; Zhang, X.; Huang, H.; Bouvier, J.; Francisco, B.; Vetting, M.; Al-Obaidi, N.; Bonanno, J.; Ghosh, A.; Zallot, R.; Andersen, H.; Almo, S.; Gerlt, J.
Functional assignment of multiple catabolic pathways for D-apiose article (2018), Nat. Chem. Biol., 14, 696-705 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
D-apiofuranose + NAD+	Agrobacterium tumefaciens	-	D-apionolactone + NADH + H+	-	r
D-apiofuranose + NAD+	Agrobacterium tumefaciens K84	-	D-apionolactone + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Agrobacterium tumefaciens	B9JK80	-	-
Agrobacterium tumefaciens K84	B9JK80	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography	Agrobacterium tumefaciens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-apiofuranose + NAD+	-	Agrobacterium tumefaciens	D-apionolactone + NADH + H+	-	r
D-apiofuranose + NAD+	-	Agrobacterium tumefaciens K84	D-apionolactone + NADH + H+	-	r

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Agrobacterium tumefaciens
NADH	-	Agrobacterium tumefaciens

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Release 2023.1 (January 2023)