Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SIRT3 | under calorie restriction, mitochondrial deacetylase Sirt3 deacetylates and activates isoform IDH2 | Mus musculus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli KRX cells | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
wild type and mutant enzyme K256Q, sitting drop vapor diffusion method, using 0.1 M sodium citrate tribasic dehydrate (pH 5.6), 11.5% (v/v) 2-propanol and 10.8% (w/v) PEG 4000 | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
K106Q | the activity of the mutant is about 118% of the wild type value | Mus musculus |
K155Q | the activity of the mutant is about 110% of the wild type value | Mus musculus |
K166Q | the activity of the mutant is about 80% of the wild type value | Mus musculus |
K180Q | the activity of the mutant is about 60% of the wild type value | Mus musculus |
K243Q | the activity of the mutant is about 90% of the wild type value | Mus musculus |
K251Q | inactive | Mus musculus |
K256Q | the activity of the mutant is about 40% of the wild type value | Mus musculus |
K263Q | the activity of the mutant is about 80% of the wild type value | Mus musculus |
K272Q | the activity of the mutant is about 65% of the wild type value | Mus musculus |
K275Q | the activity of the mutant is about 85% of the wild type value | Mus musculus |
K282Q | the activity of the mutant is about 105% of the wild type value | Mus musculus |
K360Q | the mutant shows wild type activity | Mus musculus |
K384Q | the activity of the mutant is about 90% of the wild type value | Mus musculus |
K413Q | the activity of the mutant is about 20% of the wild type value | Mus musculus |
K442Q | the activity of the mutant is about 90% of the wild type value | Mus musculus |
K80Q | the activity of the mutant is about 105% of the wild type value | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0148 | - |
NADP+ | mutant K256Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
0.0304 | - |
isocitrate | mutant K256Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
0.0337 | - |
NADP+ | wild type isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
0.0544 | - |
isocitrate | wild type isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
0.0555 | - |
isocitrate | mutant K413Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
0.1122 | - |
NADP+ | mutant K413Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Mus musculus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1 mM used in assay conditions | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | Mus musculus | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | P54071 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Resource S column chromatography | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
isocitrate + NADP+ | - |
Mus musculus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
IDH2 | - |
Mus musculus |
isocitrate dehydrogenase 2 | - |
Mus musculus |
NADP+-dependent IDH | - |
Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.66 | - |
isocitrate | mutant K256Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
3.13 | - |
NADP+ | mutant K256Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
4.61 | - |
isocitrate | mutant K413Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
5.6 | - |
isocitrate | wild type isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
6.36 | - |
NADP+ | wild type isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
7.69 | - |
NADP+ | mutant K413Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Mus musculus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
59 | - |
NADP+ | mutant K413Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
83 | - |
isocitrate | mutant K413Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
88 | - |
isocitrate | mutant K256Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
111 | - |
isocitrate | wild type isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
186 | - |
NADP+ | wild type isoform IDH2, at pH 7.4 and 25°C | Mus musculus | |
205 | - |
NADP+ | mutant K256Q of isoform IDH2, at pH 7.4 and 25°C | Mus musculus |