Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3) RIL cells | Leishmania mexicana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
NADP+ | at pH 7.4 and 37°C | Leishmania mexicana | |
0.043 | - |
DL-isocitrate | at pH 7.4 and 37°C | Leishmania mexicana |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | Km of 0.002 mM, 0.5 mM used in assay conditions | Leishmania mexicana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NADP+ | Leishmania mexicana | - |
2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania mexicana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Sephacryl-S200 gel filtration | Leishmania mexicana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
promastigote | - |
Leishmania mexicana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NADP+ | - |
Leishmania mexicana | 2-oxoglutarate + CO2 + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48100, calculated from amino acid sequence | Leishmania mexicana |
Synonyms | Comment | Organism |
---|---|---|
IDH | - |
Leishmania mexicana |
IDH-90 | - |
Leishmania mexicana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83.03 | - |
DL-isocitrate | at pH 7.4 and 37°C | Leishmania mexicana | |
84.29 | - |
NADP+ | at pH 7.4 and 37°C | Leishmania mexicana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the enzyme isoform IDH-90 exclusively catalyzes the reduction of NADP+ | Leishmania mexicana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leishmania mexicana | calculated from amino acid sequence | - |
8 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1930 | - |
DL-isocitrate | at pH 7.4 and 37°C | Leishmania mexicana | |
5620 | - |
NADP+ | at pH 7.4 and 37°C | Leishmania mexicana |