Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.42 extracted from

  • Giordana, L.; Nowicki, C.
    Two phylogenetically divergent isocitrate dehydrogenases are encoded in Leishmania parasites. Molecular and functional characterization of Leishmania mexicana isoenzymes with specificity towards NAD+ and NADP+ (2020), Mol. Biochem. Parasitol., 240, 111320 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3) RIL cells Leishmania mexicana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
NADP+ at pH 7.4 and 37°C Leishmania mexicana
0.043
-
DL-isocitrate at pH 7.4 and 37°C Leishmania mexicana

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ Km of 0.002 mM, 0.5 mM used in assay conditions Leishmania mexicana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
DL-isocitrate + NADP+ Leishmania mexicana
-
2-oxoglutarate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Leishmania mexicana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Sephacryl-S200 gel filtration Leishmania mexicana

Source Tissue

Source Tissue Comment Organism Textmining
promastigote
-
Leishmania mexicana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-isocitrate + NADP+
-
Leishmania mexicana 2-oxoglutarate + CO2 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
? x * 48100, calculated from amino acid sequence Leishmania mexicana

Synonyms

Synonyms Comment Organism
IDH
-
Leishmania mexicana
IDH-90
-
Leishmania mexicana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
83.03
-
DL-isocitrate at pH 7.4 and 37°C Leishmania mexicana
84.29
-
NADP+ at pH 7.4 and 37°C Leishmania mexicana

Cofactor

Cofactor Comment Organism Structure
NADP+ the enzyme isoform IDH-90 exclusively catalyzes the reduction of NADP+ Leishmania mexicana

pI Value

Organism Comment pI Value Maximum pI Value
Leishmania mexicana calculated from amino acid sequence
-
8

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1930
-
DL-isocitrate at pH 7.4 and 37°C Leishmania mexicana
5620
-
NADP+ at pH 7.4 and 37°C Leishmania mexicana