Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.42 extracted from

  • Niazi, A.; Bariat, L.; Riondet, C.; Carapito, C.; Mhamdi, A.; Noctor, G.; Reichheld, J.
    Cytosolic isocitrate dehydrogenase from Arabidopsis thaliana is regulated by glutathionylation (2019), Antioxidants (Basel), 8, 16 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 cells Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
C363S the mutation does not perturb the enzymatic characteristics of the enzyme. However, the mutant is less affected by nitrosoglutathione treatment than the wild type enzyme Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
GSSG increasing GSSG concentrations progressively inhibit the enzyme activity, almost complete inhibition at 1 mM Arabidopsis thaliana
additional information increasing concentrations of H2O2 hardly affect the enzyme activity Arabidopsis thaliana
nitrosoglutathione increasing nitrosoglutathione concentrations progressively inhibit the enzyme activity, complete inhibition at 2 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
NADP+ wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
0.095
-
isocitrate mutant enzyme C3636S, at pH 7.5 and 25°C Arabidopsis thaliana
0.099
-
isocitrate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Arabidopsis thaliana 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 5 mM used in assay conditions Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Arabidopsis thaliana
-
2-oxoglutarate + CO2 + NADPH + H+
-
r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9SRZ6 ecotype Columbia-0
-

Oxidation Stability

Oxidation Stability Organism
enzyme activity is affected by oxidative agents like oxidized glutathione and nitrosoglutathione through modifications of conserved Cys residues in the protein Arabidopsis thaliana

Posttranslational Modification

Posttranslational Modification Comment Organism
glutathionylation S-glutathionylation of Cys363 is involved in the inhibition of the cytosolic enzyme activity upon nitrosoglutathione treatments Arabidopsis thaliana
S-nitrosylation
-
Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
Ni2+-Sepharose column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Arabidopsis thaliana 2-oxoglutarate + CO2 + NADPH + H+
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 48300, His-tagged enzyme, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
cICDH
-
Arabidopsis thaliana
NADP-dependent isocitrate dehydrogenase
-
Arabidopsis thaliana
NADP-ICDH
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.31
-
isocitrate mutant enzyme C3636S, at pH 7.5 and 25°C Arabidopsis thaliana
4.93
-
isocitrate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana
5.81
-
NADP+ wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
7.5 8.5 high activity between pH 7.5 and 8.5 Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
45.4
-
isocitrate mutant enzyme C3636S, at pH 7.5 and 25°C Arabidopsis thaliana
49.7
-
isocitrate wild type enzyme, at pH 7.5 and 25°C Arabidopsis thaliana