Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | - |
Rattus norvegicus | |
citrate | - |
Rattus norvegicus | |
Fe2+ | the inhibitory effect of the Fe2+ and H2O2 mixture associated with the generation of hydroxyl radicals is lower in enzyme from ischemic heart compared to enzyme from normoxic heart | Rattus norvegicus | |
glutathione | - |
Rattus norvegicus | |
H2O2 | the inhibitory effect of the Fe2+ and H2O2 mixture associated with the generation of hydroxyl radicals is lower in enzyme from ischemic heart compared to enzyme from normoxic heart | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
DL-isocitrate | enzyme from ischemic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.017 | - |
isocitrate | pH 7.8, 25°C, enzyme from ischemic heart | Rattus norvegicus | |
0.045 | - |
DL-isocitrate | enzyme from normoxic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.045 | - |
isocitrate | pH 7.8, 25°C, enzyme from normoxic heart | Rattus norvegicus | |
0.046 | - |
NADP+ | enzyme from ischemic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.046 | - |
NADP+ | enzyme from normoxic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.046 | - |
NADP+ | pH 7.8, 25°C, enzyme from ischemic heart | Rattus norvegicus | |
0.046 | - |
NADP+ | pH 7.8, 25°C, enzyme from normoxic heart | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rattus norvegicus | 5829 | - |
cytosol | increase in enzyme activity during ischemia | Rattus norvegicus | 5829 | - |
mitochondrion | - |
Rattus norvegicus | 5739 | - |
mitochondrion | increase in enzyme activity during ischemia, enzyme from ischemic heart mitochondria demonstrates higher activation energy and lower thermal stability and differs in KM-value and regulation | Rattus norvegicus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | KM-value for enzyme from normoxic heart, 0.42 mM, for enzyme from ischemic heart 0.15 mM | Rattus norvegicus | |
Mn2+ | Km-value: 0.42 mM for enzyme from normoxic heart, 0.15 mM for enzyme from ischemic heart | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Rattus norvegicus | P56574 | albino | - |
Purification (Comment) | Organism |
---|---|
NADP-isocitrate dehydrogenase from the normoxic and ischemic rat myocardium | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
heart | regulation of enzyme during heart ischemia. Ischemia results in increase in enzyme activity, enzyme from ischemic heart mitochondria demonstrates higher activation energy and lower thermal stability and differs in KM-value and regulation | Rattus norvegicus | - |
heart | increase of enzyme activity in the cytosol and mitochondria of ischemic heart | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | 8 | - |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DL-isocitrate + NADP+ | - |
Rattus norvegicus | 2-oxoglutarate + CO2 + NADPH | - |
? | |
isocitrate + NADP+ | - |
Rattus norvegicus | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADP-ICDH | - |
Rattus norvegicus |
NADP-isocitrate dehydrogenase | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
enzyme from ischemic heart | Rattus norvegicus |
50 | - |
enzyme from normoxic heart | Rattus norvegicus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | 25°C: about 50% of maximal activity, 50°C: about 70% of maximal activity, enzyme from ischemic heart | Rattus norvegicus |
35 | 70 | 35°C: about 45% of maximal activity, 70°C: about 60% of maximal activity, enzyme from normoxic heart | Rattus norvegicus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
Fe2+ | enzyme from ischemic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.05 | - |
Fe2+ | enzyme from normoxic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.05 | - |
Fe2+ | pH 7.8, 25°C, enzyme from normoxic heart | Rattus norvegicus | |
0.05 | - |
Fe2+ | pH 7.8, 25°C,enzyme from ischemic heart | Rattus norvegicus | |
0.08 | - |
2-oxoglutarate | enzyme from normoxic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.08 | - |
2-oxoglutarate | pH 7.8, 25°C,enzyme from normoxic heart | Rattus norvegicus | |
0.15 | - |
glutathione | enzyme from normoxic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.15 | - |
glutathione | pH 7.8, 25°C, enzyme from normoxic heart | Rattus norvegicus | |
0.159 | - |
citrate | enzyme from normoxic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.159 | - |
citrate | pH 7.8, 25°C, enzyme from normoxic heart | Rattus norvegicus | |
0.25 | - |
2-oxoglutarate | enzyme from ischemic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.25 | - |
2-oxoglutarate | pH 7.8, 25°C,enzyme from ischemic heart | Rattus norvegicus | |
0.28 | - |
citrate | enzyme from ischemic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.28 | - |
citrate | pH 7.8, 25°C,enzyme from ischemic heart | Rattus norvegicus | |
0.45 | - |
H2O2 | enzyme from normoxic heart, pH 7.8, 25°C | Rattus norvegicus | |
0.45 | - |
H2O2 | pH 7.8, 25°C, enzyme from normoxic heart | Rattus norvegicus | |
1.63 | - |
H2O2 | enzyme from ischemic heart, pH 7.8, 25°C | Rattus norvegicus | |
1.63 | - |
H2O2 | pH 7.8, 25°C,enzyme from ischemic heart | Rattus norvegicus |