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Literature summary for 1.1.1.42 extracted from

  • Ceccarelli, C.; Grodsky, N.B.; Ariyaratne, N.; Colman, R.F.; Bahnson, B.J.
    Crystal structure of porcine mitochondrial NADP+-dependent isocitrate dehydrogenase complexed with Mn2+ and isocitrate. Insights into the enzyme mechanism (2002), J. Biol. Chem., 277, 43454-43462.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as maltose binding fusion protein, wild-type and selenomethionine enzyme variants Sus scrofa

Crystallization (Commentary)

Crystallization (Comment) Organism
20 mg/ml purified recombinant wild-type and selenomethionine enzyme, complexed with Mn2+ and isocitrate, 4°C, hanging drop vapour diffusion method, for the wild-type enzyme: 0.002 ml of enzyme solution containing 0.1 M triethanolamine chloride, pH 7.7, 0.15 M Na2SO4, 8 mM DL-isocitrate, 4 mM MnSO4, plus equal volume of 20% PEG 6000, 3% glycerol, against 0.75 ml reservoir solution containing 0.1 M triethanolamine chloride, pH 7.7, 0.15 M Na2SO4, 8 mM DL-isocitrate, 4 mM MnSO4, and 40% w/v xylitol, for the selenomethionine enzyme: 0.002 ml of enzyme solution containing 0.1 M triethanolamine chloride, pH 7.7, 0.15 M Na2SO4, 8 mM DL-isocitrate, plus equal volume of 18% PEG 6000, 3% glycerol, against 0.75 ml reservoir solution containing 0.1 M triethanolamine chloride, pH 7.7, 0.15 M Na2SO4, and 18% PEG 6000, 7-10 days, X-ray diffraction structure determination and analysis at 2.7 A resolution, modeling Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Sus scrofa 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Sus scrofa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
2 * 47000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isocitrate + NADP+ Sus scrofa
-
2-oxoglutarate + CO2 + NADPH + H+
-
?

Organism

Organism UniProt Comment Textmining
Sus scrofa P33198
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme variants fused to the maltose binding fusion protein, from Escherichia coli, to homogeneity by amylose affinity, and ion exchange chromatography Sus scrofa

Reaction

Reaction Comment Organism Reaction ID
isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH + H+ active site structure contains a well-ordered Mn2+-isocitrate complex, reaction mechanism Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
heart
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isocitrate + NADP+
-
Sus scrofa 2-oxoglutarate + CO2 + NADPH + H+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 47000, SDS-PAGE Sus scrofa

Synonyms

Synonyms Comment Organism
NADP+-dependent isocitrate dehydrogenase
-
Sus scrofa
NADP-IDH
-
Sus scrofa

Cofactor

Cofactor Comment Organism Structure
NADP+ dependent on Sus scrofa