Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | applied oxidative stress leads to upregulation of enzyme expression in erythrocytic stages | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression as Strep-tagged protein in Escherichia coli BLR(DE3) exclusively in inclusion bodies, removal of 27 amino acids from the N-terminus results in expression of partially soluble protein | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
Mg2+ | recombinant enzyme, pH 8.0, 30°C | Plasmodium falciparum | |
0.022 | - |
Mn2+ | recombinant enzyme, pH 8.0, 30°C | Plasmodium falciparum | |
0.04 | - |
D-isocitrate | recombinant enzyme, pH 8.0, 30°C | Plasmodium falciparum | |
0.09 | - |
NADP+ | recombinant enzyme, pH 8.0, 30°C | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Plasmodium falciparum | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | dependent on divalent metal ions | Plasmodium falciparum | |
Mg2+ | dependent on divalent metal ions, preferred metal ion | Plasmodium falciparum | |
Mn2+ | dependent on divalent metal ions | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
2 * 51000, about, recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
90000 | - |
recombinant enzyme, gel filtration | Plasmodium falciparum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-isocitrate + NADP+ | Plasmodium falciparum | enzyme is an integral part of the mitochondrial TCA cycle, and it is involved in providing NADPH for reductive reactions in the cell | 2-oxoglutarate + CO2 + NADPH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble enzyme from Escherichia coli | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
162 | - |
purified recombinant enzyme | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-isocitrate + NADP+ | - |
Plasmodium falciparum | 2-oxoglutarate + CO2 + NADPH | - |
? | |
D-isocitrate + NADP+ | enzyme is an integral part of the mitochondrial TCA cycle, and it is involved in providing NADPH for reductive reactions in the cell | Plasmodium falciparum | 2-oxoglutarate + CO2 + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 51000, about, recombinant enzyme, SDS-PAGE | Plasmodium falciparum |
Synonyms | Comment | Organism |
---|---|---|
ICDH | - |
Plasmodium falciparum |
isocitrate dehydrogenase | - |
Plasmodium falciparum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Plasmodium falciparum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
138 | - |
NADP+ | recombinant enzyme, pH 8.0, 30°C | Plasmodium falciparum | |
138 | - |
D-isocitrate | recombinant enzyme, pH 8.0, 30°C | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Plasmodium falciparum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | specific for, no activity with NAD+ | Plasmodium falciparum |