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Literature summary for 1.1.1.42 extracted from

  • Wrenger, C.; Mueller, S.
    Isocitrate dehydrogenase of Plasmodium falciparum. Energy metabolism or redox control? (2003), Eur. J. Biochem., 270, 1775-1783.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information applied oxidative stress leads to upregulation of enzyme expression in erythrocytic stages Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, expression as Strep-tagged protein in Escherichia coli BLR(DE3) exclusively in inclusion bodies, removal of 27 amino acids from the N-terminus results in expression of partially soluble protein Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
Mg2+ recombinant enzyme, pH 8.0, 30°C Plasmodium falciparum
0.022
-
Mn2+ recombinant enzyme, pH 8.0, 30°C Plasmodium falciparum
0.04
-
D-isocitrate recombinant enzyme, pH 8.0, 30°C Plasmodium falciparum
0.09
-
NADP+ recombinant enzyme, pH 8.0, 30°C Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Plasmodium falciparum 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ dependent on divalent metal ions Plasmodium falciparum
Mg2+ dependent on divalent metal ions, preferred metal ion Plasmodium falciparum
Mn2+ dependent on divalent metal ions Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
2 * 51000, about, recombinant enzyme, SDS-PAGE Plasmodium falciparum
90000
-
recombinant enzyme, gel filtration Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-isocitrate + NADP+ Plasmodium falciparum enzyme is an integral part of the mitochondrial TCA cycle, and it is involved in providing NADPH for reductive reactions in the cell 2-oxoglutarate + CO2 + NADPH
-
?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant soluble enzyme from Escherichia coli Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
162
-
purified recombinant enzyme Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-isocitrate + NADP+
-
Plasmodium falciparum 2-oxoglutarate + CO2 + NADPH
-
?
D-isocitrate + NADP+ enzyme is an integral part of the mitochondrial TCA cycle, and it is involved in providing NADPH for reductive reactions in the cell Plasmodium falciparum 2-oxoglutarate + CO2 + NADPH
-
?

Subunits

Subunits Comment Organism
dimer 2 * 51000, about, recombinant enzyme, SDS-PAGE Plasmodium falciparum

Synonyms

Synonyms Comment Organism
ICDH
-
Plasmodium falciparum
isocitrate dehydrogenase
-
Plasmodium falciparum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Plasmodium falciparum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
138
-
NADP+ recombinant enzyme, pH 8.0, 30°C Plasmodium falciparum
138
-
D-isocitrate recombinant enzyme, pH 8.0, 30°C Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Plasmodium falciparum

Cofactor

Cofactor Comment Organism Structure
NADP+ specific for, no activity with NAD+ Plasmodium falciparum