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show all sequences of 1.1.1.412

Functional characterization of an NADPH dependent 2-alkyl-3-ketoalkanoic acid reductase involved in olefin biosynthesis in Stenotrophomonas maltophilia

Bonnett, S.A.; Papireddy, K.; Higgins, S.; del Cardayre, S.; Reynolds, K.A.; Biochemistry 50, 9633-9640 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Stenotrophomonas maltophilia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
NADP+
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.026
-
NADPH
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.031
-
syn-2-decyl-3-hydroxytetradecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.161
-
(2R/2S)-2-hexyl-3-oxodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.181
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.202
-
syn-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.214
-
syn-2-octyl-3-hydroxydodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
77124
-
His6-tagged enzyme, calculated from amino acid sequence
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
Stenotrophomonas maltophilia
-
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
-
r
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
Stenotrophomonas maltophilia K279a
-
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
-
r
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
Stenotrophomonas maltophilia
-
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
-
r
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
Stenotrophomonas maltophilia K279a
-
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Stenotrophomonas maltophilia
B2FI29
-
-
Stenotrophomonas maltophilia K279a
B2FI29
-
-
Purification (Commentary)
Commentary
Organism
Ni2+-agarose affinity column chromatography
Stenotrophomonas maltophilia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
741882
Stenotrophomonas maltophilia
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
-
-
r
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
741882
Stenotrophomonas maltophilia K279a
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
-
-
r
(2R,3S)-2-hexyl-3-hydroxydecanoic acid + NADPH + H+
-
741882
Stenotrophomonas maltophilia
?
-
-
-
?
(2R,3S)-2-hexyl-3-hydroxydecanoic acid + NADPH + H+
-
741882
Stenotrophomonas maltophilia K279a
?
-
-
-
?
(2R/2S)-2-hexyl-3-oxodecanoic acid + NADPH + H+
13% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid
741882
Stenotrophomonas maltophilia
?
-
-
-
?
(2R/2S)-2-hexyl-3-oxodecanoic acid + NADPH + H+
13% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid
741882
Stenotrophomonas maltophilia K279a
?
-
-
-
?
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
741882
Stenotrophomonas maltophilia
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
-
-
r
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
741882
Stenotrophomonas maltophilia K279a
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
-
-
r
additional information
no activity with syn-2-butyl-3-hydroxyoctanoic acid, 3-oxodecanoic acid, and 3-hydroxydecanoic acid
741882
Stenotrophomonas maltophilia
?
-
-
-
-
additional information
no activity with syn-2-butyl-3-hydroxyoctanoic acid, 3-oxodecanoic acid, and 3-hydroxydecanoic acid
741882
Stenotrophomonas maltophilia K279a
?
-
-
-
-
syn-2-decyl-3-hydroxytetradecanoic acid + NADPH + H+
100% activity
741882
Stenotrophomonas maltophilia
?
-
-
-
?
syn-2-hexyl-3-hydroxydecanoic acid + NADPH + H+
15% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid
741882
Stenotrophomonas maltophilia
?
-
-
-
?
syn-2-octyl-3-hydroxydodecanoic acid + NADPH + H+
20% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid
741882
Stenotrophomonas maltophilia
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
homodimer
2 * 36000, SDS-PAGE; 2 * 36472, calculated from amino acid sequence
Stenotrophomonas maltophilia
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.97
-
(2R/2S)-2-hexyl-3-oxodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
1.2
-
syn-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
1.3
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
1.4
-
syn-2-decyl-3-hydroxytetradecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
2
-
syn-2-octyl-3-hydroxydodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Stenotrophomonas maltophilia
NADPH
-
Stenotrophomonas maltophilia
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Stenotrophomonas maltophilia
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Stenotrophomonas maltophilia
NADPH
-
Stenotrophomonas maltophilia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.024
-
NADP+
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.026
-
NADPH
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.031
-
syn-2-decyl-3-hydroxytetradecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.161
-
(2R/2S)-2-hexyl-3-oxodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.181
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.202
-
syn-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
0.214
-
syn-2-octyl-3-hydroxydodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
77124
-
His6-tagged enzyme, calculated from amino acid sequence
Stenotrophomonas maltophilia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
Stenotrophomonas maltophilia
-
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
-
r
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
Stenotrophomonas maltophilia K279a
-
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
-
r
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
Stenotrophomonas maltophilia
-
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
-
r
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
Stenotrophomonas maltophilia K279a
-
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
Ni2+-agarose affinity column chromatography
Stenotrophomonas maltophilia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
741882
Stenotrophomonas maltophilia
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
-
-
r
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
741882
Stenotrophomonas maltophilia K279a
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
-
-
r
(2R,3S)-2-hexyl-3-hydroxydecanoic acid + NADPH + H+
-
741882
Stenotrophomonas maltophilia
?
-
-
-
?
(2R,3S)-2-hexyl-3-hydroxydecanoic acid + NADPH + H+
-
741882
Stenotrophomonas maltophilia K279a
?
-
-
-
?
(2R/2S)-2-hexyl-3-oxodecanoic acid + NADPH + H+
13% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid
741882
Stenotrophomonas maltophilia
?
-
-
-
?
(2R/2S)-2-hexyl-3-oxodecanoic acid + NADPH + H+
13% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid
741882
Stenotrophomonas maltophilia K279a
?
-
-
-
?
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
741882
Stenotrophomonas maltophilia
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
-
-
r
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+
-
741882
Stenotrophomonas maltophilia K279a
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+
-
-
-
r
additional information
no activity with syn-2-butyl-3-hydroxyoctanoic acid, 3-oxodecanoic acid, and 3-hydroxydecanoic acid
741882
Stenotrophomonas maltophilia
?
-
-
-
-
additional information
no activity with syn-2-butyl-3-hydroxyoctanoic acid, 3-oxodecanoic acid, and 3-hydroxydecanoic acid
741882
Stenotrophomonas maltophilia K279a
?
-
-
-
-
syn-2-decyl-3-hydroxytetradecanoic acid + NADPH + H+
100% activity
741882
Stenotrophomonas maltophilia
?
-
-
-
?
syn-2-hexyl-3-hydroxydecanoic acid + NADPH + H+
15% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid
741882
Stenotrophomonas maltophilia
?
-
-
-
?
syn-2-octyl-3-hydroxydodecanoic acid + NADPH + H+
20% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid
741882
Stenotrophomonas maltophilia
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homodimer
2 * 36000, SDS-PAGE; 2 * 36472, calculated from amino acid sequence
Stenotrophomonas maltophilia
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.97
-
(2R/2S)-2-hexyl-3-oxodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
1.2
-
syn-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
1.3
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
1.4
-
syn-2-decyl-3-hydroxytetradecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
2
-
syn-2-octyl-3-hydroxydodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
General Information
General Information
Commentary
Organism
metabolism
the enzyme is involved in olefin biosynthesis
Stenotrophomonas maltophilia
General Information (protein specific)
General Information
Commentary
Organism
metabolism
the enzyme is involved in olefin biosynthesis
Stenotrophomonas maltophilia
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6
-
(2R/2S)-2-hexyl-3-oxodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
6
-
syn-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
7.3
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
9.6
-
syn-2-octyl-3-hydroxydodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
47.9
-
syn-2-decyl-3-hydroxytetradecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
6
-
(2R/2S)-2-hexyl-3-oxodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
6
-
syn-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
7.3
-
(2R,3S)-2-hexyl-3-hydroxydecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
9.6
-
syn-2-octyl-3-hydroxydodecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
47.9
-
syn-2-decyl-3-hydroxytetradecanoic acid
at pH 8.0 and 25°C
Stenotrophomonas maltophilia
Other publictions for EC 1.1.1.412
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742283
Kancharla
Stenotrophomonas maltophilia ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia K279a
ChemBioChem
17
1426-1429
2016
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741882
Bonnett
Functional characterization o ...
Stenotrophomonas maltophilia, Stenotrophomonas maltophilia K279a
Biochemistry
50
9633-9640
2011
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1
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7
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1
4
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5
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1
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13
1
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5
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2
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1
2
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7
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1
4
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1
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13
1
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5
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1
1
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5
5