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Literature summary for 1.1.1.41 extracted from

  • Soundar, S.; Ohagan, M.; Fomulu, K.S.; Colman, R.F.
    Identification of Mn2+-binding aspartates from alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase (2006), J. Biol. Chem., 281, 21073-21081.
    View publication on PubMed
Search for more literature for 1.1.1.41

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
D206N alpha-subunit mutant Homo sapiens
D206N specific activity of the alpha-subunit mutant enzyme is 1.3fold higher than specific activity of wild-type enzyme Homo sapiens
D215N gamma-subunit mutant Homo sapiens
D215N specific activity of the gamma-subunit mutant enzyme is 17% of specific activity of wild-type enzyme. 100fold increase in KM-value for Mn2+. Km-value for isocitrate is elevated Homo sapiens
D217N beta-subunit mutant Homo sapiens
D217N specific activity of the beta-subunit mutant enzyme is 33% of specific activity of wild-type enzyme. 16fold increase in KM-vlaue for NAD+ Homo sapiens
D230C alpha-subunit mutant Homo sapiens
D230C specific activity of the alpha-subunit mutant enzyme is 6% of specific activity of wild-type enzyme. 32fold increase in KM-value for Mn2+. Km-value for isocitrate is elevated. 16fold increase in KM-vlaue for NAD+ Homo sapiens
D230N alpha-subunit mutant Homo sapiens
D230N mutation in alpha-subunit results in complete loss of activity Homo sapiens
D234C alpha-subunit mutant Homo sapiens
D234C specific activity of the alpha-subunit mutant enzyme is 1% of specific activity of wild-type enzyme Homo sapiens
D234N alpha-subunit mutant Homo sapiens
D234N mutation in alpha-subunit results in complete loss of activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.04
-
 NAD+ 25°C, pH 7.2, alpha-subunit mutant enzyme D234C Homo sapiens
0.07
-
 NAD+ 25°C, pH 7.2, wild-type enzyme Homo sapiens
0.14
-
 NAD+ 25°C, pH 7.2, alpha-subunit mutant enzyme D206N Homo sapiens
0.19
-
 NAD+ 25°C, pH 7.2, gamma-subunit mutant enzyme D215N Homo sapiens
0.68
-
 NAD+ 25°C, pH 7.2, beta-subunit mutant enzyme D217N Homo sapiens
1.1
-
 NAD+ 25°C, pH 7.2, alpha-subunit mutant enzyme D230C Homo sapiens
2.2
-
 isocitrate wild type enzyme in 20 mM isocitrate and 1mM MnSO4 Homo sapiens
2.2
-
 isocitrate 25°C, pH 7.2, wild-type enzyme Homo sapiens
3.4
-
 isocitrate D234C, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4 Homo sapiens
3.4
-
 isocitrate 25°C, pH 7.2, alpha-subunit mutant enzyme D234C Homo sapiens
3.5
-
 isocitrate D206N, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4 Homo sapiens
3.5
-
 isocitrate 25°C, pH 7.2, alpha-subunit mutant enzyme D206N Homo sapiens
5.1
-
 isocitrate D217N, beta-subunit mutant, in 20 mM isocitrate and 1mM MnSO4 Homo sapiens
5.1
-
 isocitrate 25°C, pH 7.2, beta-subunit mutant enzyme D217N Homo sapiens
20
-
 isocitrate D230C, alpha-subunit mutant, in 20 mM isocitrate and 1mM MnSO4 Homo sapiens
20
-
 isocitrate 25°C, pH 7.2, alpha-subunit mutant enzyme D230C Homo sapiens
41
-
 isocitrate D215N, gamma-subunit mutant, in 20 mM isocitrate and 1mM MnSO4 Homo sapiens
41
-
 isocitrate 25°C, pH 7.2, gamma-subunit mutant enzyme D215N Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ essential divalent metal ion Homo sapiens
Mg2+ essential divalent metal ion Homo sapiens
Mn2+ essential divalent metal ion Homo sapiens
Mn2+ alpha subunit Asp230 and gamma-subunit Asp215 may interact directly with the Mn2+. KM-value: 0.22 mM for wild-type enzyme, 0.32 mM for alpha-subunit mutant enzyme D206N, 7.13 mM for alpha-subunit mutant enzyme D230C, 0.46 mM for alpha-subunit mutant enzyme D234C, 0.1 mM for beta-subunit mutant enzyme D217N, 21.1 mM for gamma-subunit mutant enzyme D215N Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37000
-
 2 * 37000, 1 * 39000, 1 * 39000 Homo sapiens
39000
-
 2 * 37000, 1 * 39000, 1 * 39000 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation and gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.2
-
 D234C, alpha-subunit mutant, in 20 mM D-isocitrate and 1 mM MnSO4 Homo sapiens
1.4
-
 D230C, alpha-subunit mutant, in 20 mM D-isocitrate and 1 mM MnSO4 Homo sapiens
3.7
-
 D215N, gamma-subunit mutant, in 20 mM D-isocitrate and 1 mM MnSO4 Homo sapiens
7.3
-
 D217N, beta-subunit mutant, in 20 mM D-isocitrate and 1 mM MnSO4 Homo sapiens
22
-
 wild type enzyme, in 20 mM D-isocitrate and 1 mM MnSO4 Homo sapiens
29
-
 D206N, alpha-subunit mutant, in 20 mM D-isocitrate and 1 mM MnSO4 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-isocitrate + NAD+
-
 Homo sapiens 2-oxoglutarate + CO2 + NADH
-
 ?
isocitrate + NAD+
-
 Homo sapiens 2-oxoglutarate + CO2 + NADH + H+
-
 ?

Subunits

Subunits Comment Organism
heterotetramer 2 * 37000, 1 * 39000, 1 * 39000 Homo sapiens

Synonyms

Synonyms Comment Organism
IDH
-
 Homo sapiens
NAD-dependent isocitrate dehydrogenase
-
 Homo sapiens
NAD-IDH
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.27
-
 D-isocitrate D234C, alpha-subunit mutant, in 20 mM isocitrate and 1 mM MnSO4 Homo sapiens
0.27
-
 NAD+ 25°C, pH 7.2, alpha-subunit mutant enzyme D234C Homo sapiens
0.27
-
 isocitrate 25°C, pH 7.2, alpha-subunit mutant enzyme D234C Homo sapiens
2.49
-
 D-isocitrate D230C, alpha-subunit mutant, in 20 mM isocitrate and 1 mM MnSO4 Homo sapiens
2.49
-
 NAD+ 25°C, pH 7.2, alpha-subunit mutant enzyme D230C Homo sapiens
2.49
-
 isocitrate 25°C, pH 7.2, alpha-subunit mutant enzyme D230C Homo sapiens
11.8
-
 D-isocitrate D215N, gamma-subunit mutant, in 20 mM isocitrate and 1 mM MnSO4 Homo sapiens
11.8
-
 NAD+ 25°C, pH 7.2, gamma-subunit mutant enzyme D215N Homo sapiens
11.8
-
 isocitrate 25°C, pH 7.2, gamma-subunit mutant enzyme D215N Homo sapiens
19.6
-
 D-isocitrate D217N, beta-subunit mutant, in 20 mM isocitrate and 1 mM MnSO4 Homo sapiens
19.6
-
 NAD+ 25°C, pH 7.2, beta-subunit mutant enzyme D217N Homo sapiens
19.6
-
 isocitrate 25°C, pH 7.2, beta-subunit mutant enzyme D217N Homo sapiens
30
-
 D-isocitrate wild type enzyme in 20 mM isocitrate and 1 mM MnSO4 Homo sapiens
30
-
 NAD+ 25°C, pH 7.2, wild-type enzyme Homo sapiens
30
-
 isocitrate 25°C, pH 7.2, wild-type enzyme Homo sapiens
36.8
-
 D-isocitrate D206N, alpha-subunit mutant, in 20 mM isocitrate and 1 mM MnSO4 Homo sapiens
36.8
-
 NAD+ 25°C, pH 7.2, alpha-subunit mutant enzyme D206N Homo sapiens
36.8
-
 isocitrate 25°C, pH 7.2, alpha-subunit mutant enzyme D206N Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens
NADH
-
 Homo sapiens