Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ADP | allosteric regulation, determination of mol ADP bound to an enzyme tetramer of wild-type and mutant enzymes at different starting concentrations, overview | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpression of the wild-type and a mutant enzyme, the latter containing the wild-type 2 subunits alpha and beta plus an additional third one, gamma, overexpression of the 3-subunit mutant and the same mutant with point mutations of Arg-residues in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R88Q | site-directed mutagenesis, residue of the alpha-subunit, inactive mutant | Homo sapiens |
R97Q | site-directed mutagenesis, residue of the additional mutant gamma-subunit, highly reduced activity compared to the wild-type enzyme | Homo sapiens |
R99Q | site-directed mutagenesis, residue of the beta-subunit, reduced activity compared to the wild-type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NAD+ | purified recombinant mutant gamma-R97Q, pH 7.2, 25°C | Homo sapiens | |
0.014 | - |
NAD+ | purified recombinant mutant beta-R99Q, pH 7.2, 25°C | Homo sapiens | |
0.073 | - |
NAD+ | purified recombinant wild-type enzyme, pH 7.2, 25°C | Homo sapiens | |
0.22 | - |
Mn2+ | purified recombinant wild-type enzyme and mutant beta-R99Q, pH 7.2, 25°C | Homo sapiens | |
0.32 | - |
isocitrate | purified recombinant wild-type enzyme, pH 7.2, 25°C, in presence of 1 mM ADP | Homo sapiens | |
0.39 | - |
Mn2+ | purified recombinant mutant gamma-R97Q, pH 7.2, 25°C | Homo sapiens | |
0.77 | - |
isocitrate | purified recombinant mutant beta-R99Q, pH 7.2, 25°C, in presence of 1 mM ADP | Homo sapiens | |
0.9 | - |
isocitrate | purified recombinant mutant beta-R99Q, pH 7.2, 25°C | Homo sapiens | |
1.8 | - |
isocitrate | purified recombinant mutant gamma-R97Q, pH 7.2, 25°C, in presence of 1 mM ADP | Homo sapiens | |
2 | - |
isocitrate | purified recombinant mutant gamma-R97Q, pH 7.2, 25°C | Homo sapiens | |
2.2 | - |
isocitrate | purified recombinant wild-type enzyme, pH 7.2, 25°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
315000 | - |
about, recombinant wild-type and mutant enzymes, gel filtration | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
threo-Ds-isocitrate + NAD+ | Homo sapiens | - |
2-oxoglutarate + CO2 + NADH | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant enzymes from Escherichia coli | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
isocitrate + NAD+ = 2-oxoglutarate + CO2 + NADH + H+ | Arg97, and Arg99 contribute to activity, Arg88 is essential for catalysis | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.2 | - |
purified recombinant mutant gamma-R97Q | Homo sapiens |
13.5 | - |
purified recombinant mutant beta-R99Q | Homo sapiens |
21.7 | - |
purified recombinant wild-type enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
threo-Ds-isocitrate + NAD+ | - |
Homo sapiens | 2-oxoglutarate + CO2 + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme subunit ratio of recombinant wild-type and mutant enzymes, overview | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
NAD-dependent isocitrate dehydrogenase | - |
Homo sapiens |
threo-DS-isocitrate:NAD+ oxidoreductase (decarboxylating) | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | specific for | Homo sapiens |