General Stability | Organism |
---|---|
sucrose stabilizes enzyme preparation | Cereibacter sphaeroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,2'-dipyridyl | 13% inhibition at 1 mM, restored by addition of 2 mM MgCl2 | Cereibacter sphaeroides | |
EDTA | 68% inhibition at 1 mM, restored by addition of 2 mM MgCl2 | Cereibacter sphaeroides | |
FeSO4 | - |
Cereibacter sphaeroides | |
ZnSO4 | - |
Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
NADH | - |
Cereibacter sphaeroides | |
0.012 | - |
NAD+ | - |
Cereibacter sphaeroides | |
0.2 | - |
1,2-hexanediol | - |
Cereibacter sphaeroides | |
48 | - |
dihydroxyacetone | - |
Cereibacter sphaeroides | |
62 | - |
acetoin | - |
Cereibacter sphaeroides | |
85 | - |
D-threitol | - |
Cereibacter sphaeroides | |
144 | - |
L-erythrulose | - |
Cereibacter sphaeroides | |
240 | - |
galactitol | - |
Cereibacter sphaeroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 21% activation at 1 mM | Cereibacter sphaeroides | |
Mg2+ | 1 mM required for optimal activity | Cereibacter sphaeroides | |
Mn2+ | 1 mM required for optimal activity | Cereibacter sphaeroides | |
Ni2+ | 25% activation at 1 mM | Cereibacter sphaeroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
4 * 28000, SDS-PAGE | Cereibacter sphaeroides |
110000 | - |
sucrose density gradient centrifugation | Cereibacter sphaeroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitol + NAD+ | Cereibacter sphaeroides | - |
? + NADH | - |
? | |
D-ribulose + NADH | Cereibacter sphaeroides | - |
? + NAD+ | - |
? | |
D-threitol + NAD+ | Cereibacter sphaeroides | best substrate tested | ? + NADH | - |
? | |
D-xylulose + NADH | Cereibacter sphaeroides | - |
? + NAD+ | - |
? | |
galactitol + NAD+ | Cereibacter sphaeroides | - |
L-tagatose + NADH | - |
r | |
L-erythrulose + NADH | Cereibacter sphaeroides | - |
? + NAD+ | - |
? | |
L-glucitol + NAD+ | Cereibacter sphaeroides | - |
? + NADH | - |
? | |
xylitol + NAD+ | Cereibacter sphaeroides | high activity | L-xylulose + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Cereibacter sphaeroides |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Cereibacter sphaeroides | - |
Storage Stability | Organism |
---|---|
4°C, 20 mM Bis-Tris, pH 6.5, 1 mM MgCl2, 30% sucrose, stable for 3 months | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R)-butanol + NAD+ | - |
Cereibacter sphaeroides | 2-butanone + NADH | - |
? | |
(2S)-butanol + NAD+ | - |
Cereibacter sphaeroides | 2-butanone + NADH | - |
? | |
(2S,3S)-butanediol + NAD+ | - |
Cereibacter sphaeroides | ? + NADH | - |
? | |
1,2-butanediol + NAD+ | - |
Cereibacter sphaeroides | ? + NADH | - |
? | |
1,2-hexanediol + NAD+ | - |
Cereibacter sphaeroides | ? + NADH | - |
? | |
1,2-pentanediol + NAD+ | - |
Cereibacter sphaeroides | ? + NADH | - |
? | |
1,2-propanediol + NAD+ | - |
Cereibacter sphaeroides | ? + NADH | - |
? | |
2-hexanol + NAD+ | - |
Cereibacter sphaeroides | 2-hexanone + NADH | - |
? | |
2-propanol + NAD+ | - |
Cereibacter sphaeroides | acetone + NADH | - |
? | |
acetoin + NADH | - |
Cereibacter sphaeroides | ? + NAD+ | - |
? | |
D-arabitol + NAD+ | - |
Cereibacter sphaeroides | ? + NADH | - |
? | |
D-ribulose + NADH | - |
Cereibacter sphaeroides | ? + NAD+ | - |
? | |
D-threitol + NAD+ | best substrate tested | Cereibacter sphaeroides | ? + NADH | - |
? | |
D-xylulose + NADH | - |
Cereibacter sphaeroides | ? + NAD+ | - |
? | |
dihydroxyacetone + NADH | - |
Cereibacter sphaeroides | ? + NAD+ | - |
? | |
galactitol + NAD+ | - |
Cereibacter sphaeroides | L-tagatose + NADH | - |
r | |
glycerol + NAD+ | - |
Cereibacter sphaeroides | DL-glyceraldehyde + NADH | - |
r | |
L-erythrulose + NADH | - |
Cereibacter sphaeroides | ? + NAD+ | - |
? | |
L-glucitol + NAD+ | - |
Cereibacter sphaeroides | ? + NADH | - |
? | |
xylitol + NAD+ | high activity | Cereibacter sphaeroides | L-xylulose + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 28000, SDS-PAGE | Cereibacter sphaeroides |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
50% loss of activity after 8 days | Cereibacter sphaeroides |
50 | - |
50% loss of activity after 12 min | Cereibacter sphaeroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
reduction of acetoin | Cereibacter sphaeroides |
10.5 | - |
1,2-hexanediol oxidation | Cereibacter sphaeroides |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
63% of the reduction activity | Cereibacter sphaeroides |
9 | - |
81% of the oxidation activity | Cereibacter sphaeroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | strictly specific | Cereibacter sphaeroides | |
NADH | strictly specific | Cereibacter sphaeroides |